Koubek K G, Ussary J P, Haulsee R E
J Assoc Off Anal Chem. 1979 Nov;62(6):1297-301.
An HPLC method was developed to determine residues of individual isomers of brodifacoum (3-[3-4'-bromo[1,1'-biphenyl]-4-yl)-1, 2, 3, 4-tetrahydro-1-naphthalenyl]-4-hydroxy-2H-1-benzopyran-2-one) in rat tissue. The compound was extracted twice with 10% methanol in chloroform, filtered, and cleaned up by using automated gel permeation chromatography. A final cleanup on a silica gel SEP-PAK was added to protect the analytical column from irreversible adsorption and to reduce analysis time. The analysis was done on a microPorasil column; a UV detector was used for quantitation. Recoveries of brodifacoum added to rat tissue in concentrations of 0.12-5.0 ppm were greater than 90%.
建立了一种高效液相色谱法（HPLC），用于测定大鼠组织中溴敌隆（3 - [3 - （4'-溴联苯 - 4 - 基） - 1,2,3,4 - 四氢 - 1 - 萘基] - 4 - 羟基 - 2H - 1 - 苯并吡喃 - 2 - 酮）各异构体的残留量。该化合物用10%甲醇的氯仿溶液萃取两次，过滤后，通过自动凝胶渗透色谱法进行净化。最后在硅胶SEP - PAK上进行净化，以保护分析柱免受不可逆吸附并减少分析时间。分析在微孔硅胶柱上进行；使用紫外检测器进行定量。添加到大鼠组织中浓度为0.12 - 5.0 ppm的溴敌隆回收率大于90%。
