Gardner A M
J Assoc Off Anal Chem. 1979 Nov;62(6):1315-8.
The flame retardant tris-(2,3-dibromopropyl) phosphate (Tris) was chromatographed on silica gel thin layer plates, using ethyl acetate-hexane (30+70) as the developing solvent. Tris was visualized by spraying the chromatogram with 1% aqueous silver nitrate followed by exposure to ultraviolet light for 40 min. Tris appeared as dark gray spots on a white background; the spots were quantitated by densitometry at 660 nm. The lower level of sensitivity was 50 ng; calibration plots were linear from 50 to 800 ng. The procedure was developed to determine the levels of unmetabolized Tris remaining after exposure to sewage sludge suspensions. Recovery of Tris from 3 sewage sludge samples fortified at the 1.0 ppm level averaged 97%.
将阻燃剂磷酸三（2,3 - 二溴丙基）酯（Tris）在硅胶薄层板上进行色谱分析，使用乙酸乙酯 - 己烷（30 + 70）作为展开溶剂。通过用1%的硝酸银水溶液喷雾色谱图，然后在紫外线下照射40分钟来使Tris可视化。Tris在白色背景上呈现为深灰色斑点；通过在660nm处进行光密度测定对斑点进行定量。最低检测限为50ng；校准曲线在50至800ng范围内呈线性。开发该程序是为了测定暴露于污水污泥悬浮液后残留的未代谢Tris的水平。在1.0ppm水平加标的3个污水污泥样品中Tris的平均回收率为97%。
