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ATP诱导的扇贝闭壳肌肌球蛋白的差示紫外吸收光谱

Difference UV-absorption spectrum of scallop adductor myosin induced by ATP.

作者信息

Kondo S, Asakawa T, Morita F

出版信息

J Biochem. 1979 Nov;86(5):1567-71. doi: 10.1093/oxfordjournals.jbchem.a132674.

Abstract

A difference UV-absorption spectrum induced by ATP was observed with scallop myosins purified from both striated and smooth adductor muscles. The difference spectra showed a positive peak at 289 nm with a shoulder around 295 nm, and two small negative troughs around 280 nm. Tryptophanyl movement similar to that in rabbit skeletal myosin is indicated. Some tyrosyl movements in scallop myosins, however, may be in the opposite direction.

摘要

从横纹肌和平滑肌闭壳肌中纯化得到的扇贝肌球蛋白,观察到了由ATP诱导产生的差示紫外吸收光谱。差示光谱在289nm处有一个正峰,在295nm左右有一个肩峰,在280nm左右有两个小的负谷。这表明存在类似于兔骨骼肌肌球蛋白中的色氨酸移动。然而,扇贝肌球蛋白中的一些酪氨酸移动可能方向相反。

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