Barry A L, Coyle M B, Thornsberry C, Gerlach E H, Hawkinson R W
J Clin Microbiol. 1979 Dec;10(6):885-9. doi: 10.1128/jcm.10.6.885-889.1979.
Standard Bauer-Kirby disk tests were performed with 85 selected isolates, each tested in triplicate by four different investigators. Each disk test was observed, and zone diameters were measured, under two lighting conditions (transmitted light and reflected light). The two lighting systems produced similar zone measurements (+/-2 mm) with 96% of the tests. When there were greater differences, zones appeared to be larger when observed with reflected light. Interlaboratory reproducibility was much greater when using reflected light rather than transmitted light. We concluded that zone diameters should be measured from the back of the plate while it is resting on, or held 2 to 3 inches [ca. 5.1 to 7.6 cm] above, a black, nonreflecting, flat surface, illuminated by a reflected light source.
对85株选定的分离株进行了标准的鲍尔-柯比纸片扩散试验，由四名不同的研究人员对每株分离株进行三次重复测试。在两种光照条件下（透射光和反射光）观察每次纸片扩散试验，并测量抑菌圈直径。在96%的试验中，两种光照系统产生的抑菌圈测量结果相似（±2毫米）。当差异较大时，用反射光观察时抑菌圈似乎更大。使用反射光时实验室间的重复性比使用透射光时要高得多。我们得出结论，应在平板置于黑色、无反射的平面上或距该平面2至3英寸（约5.1至7.6厘米）高处，由反射光源照明时，从平板背面测量抑菌圈直径。
