Shu S, Deng J S, Vetter S, Beutner E H
Ann N Y Acad Sci. 1975 Jun 30;254:559-72. doi: 10.1111/j.1749-6632.1975.tb29203.x.
The effect of conjugate F/P ratios on titers of patient antibodies was evaluated with two model systems in IIF chessboard titrations, notably, antinuclear antibodies and the intercellular antibodies of pemphigus. The plateau titers of both antibodies and F/P conjugate ratios were analyzed and proved to have a high degree of linear relationship statistically. Single titrations of other tissue antibodies, including skin basement membrane antibodies of bullous pemphigoid, MTA, GPA, SMA, and ThA with the same group of conjugates yielded similar information. A quantitative relationship between the two parameters was established.
在间接免疫荧光棋盘滴定法中，使用两个模型系统评估了共轭物的F/P比率对患者抗体滴度的影响，具体而言，即抗核抗体和天疱疮的细胞间抗体。分析了两种抗体的平台滴度与F/P共轭比率，并证明在统计学上具有高度的线性关系。用同一组共轭物对其他组织抗体进行单次滴定，包括大疱性类天疱疮的皮肤基底膜抗体、MTA、GPA、SMA和ThA，也得到了类似的结果。建立了这两个参数之间的定量关系。
