用于衣原体抗原荧光抗体偶联物的山羊血清。
Goat serums for fluorescent antibody conjugates to chlamydial antigens.
作者信息
Tessler J
出版信息
Can J Comp Med. 1984 Apr;48(2):228-9.
Serums from goats hyperimmunized with Chlamydia psittaci consistently produce antichlamydial fluorescent antibody conjugate of high titer. The titer of the fluorescent antibody conjugate prepared from a given serum correlated well with the titer obtained by agar gel precipitin, but not with the complement fixation. The agar gel precipitin test can be used to predict whether a given serum is satisfactory for use in production of a conjugate for direct fluorescent antibody tests. Serums with an agar gel precipitin titer of 1/8 or higher generally produce a usable fluorescent antibody conjugate. Labeling gamma globulins with fluorescein isothiocyanate at a ratio of 1/150 resulted in satisfactory fluorescent antibody conjugates. Cultures of Vero cells infected with chlamydiae were found to be suitable for titration of the fluorescent antibody conjugates.
用鹦鹉热衣原体超免疫的山羊血清始终能产生高滴度的抗衣原体荧光抗体结合物。从给定血清制备的荧光抗体结合物的滴度与琼脂凝胶沉淀素法测得的滴度相关性良好，但与补体结合试验测得的滴度无关。琼脂凝胶沉淀素试验可用于预测给定血清是否适合用于制备直接荧光抗体试验的结合物。琼脂凝胶沉淀素滴度为1/8或更高的血清通常能产生可用的荧光抗体结合物。以1/150的比例用异硫氰酸荧光素标记γ球蛋白可得到令人满意的荧光抗体结合物。发现感染衣原体的Vero细胞培养物适合用于滴定荧光抗体结合物。
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