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1
Goat serums for fluorescent antibody conjugates to chlamydial antigens.用于衣原体抗原荧光抗体偶联物的山羊血清。
Can J Comp Med. 1984 Apr;48(2):228-9.
2
Direct immunofluorescence tests with counterstains for detection of Chlamydia psittaci in infected avian tissues.采用复染剂的直接免疫荧光试验检测感染禽类组织中的鹦鹉热衣原体。
Can J Comp Med. 1979 Apr;43(2):217-22.
3
Comparison of direct and modified direct complement-fixation and agar-gel precipitin methods in detecting chlamydial antibody in wild birds.直接法、改良直接补体结合法和琼脂凝胶沉淀法检测野生鸟类衣原体抗体的比较
Avian Dis. 1978 Jul-Sep;22(3):422-30.
4
[Chlamydial infection in mass abortions in goats].[山羊大规模流产中的衣原体感染]
Vet Med Nauki. 1984;21(5):33-40.
5
An enzyme-linked immunosorbent assay (ELISA) for the detection of chlamydial antibodies in caprine sera.一种用于检测山羊血清中衣原体抗体的酶联免疫吸附测定（ELISA）。
Ann Rech Vet. 1985;16(4):393-8.
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Tierarztl Prax. 1991 Dec;19(6):630-4.
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[Production of labelled gamma globulin for the detection of Chlamydia].[用于检测衣原体的标记γ球蛋白的制备]
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DIRECT AND INDIRECT FLUORESCENT-ANTIBODY TECHNIQUES FOR THE PSITTACOSIS-LYMPHOGRANULOMA VENEREUM-TRACHOMA GROUP OF AGENTS.鹦鹉热-性病性淋巴肉芽肿-沙眼菌群病原体的直接和间接荧光抗体技术
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本文引用的文献

1
Effects of cortisone, cytochalasin B and cycloheximide on strains of Chlamydia psittaci in cell cultures.可的松、细胞松弛素B和放线菌酮对细胞培养中鹦鹉热衣原体菌株的影响。
Can J Comp Med. 1983 Jul;47(3):352-7.
2
Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation.异硫氰酸荧光素与抗体的结合。I. 结合条件的实验。
Immunology. 1970 Jun;18(6):865-73.
3
Fluorescent labeling of antibody for identifying mycoplasma colonies by incident ultraviolet light.用于通过入射紫外光鉴定支原体菌落的抗体荧光标记。
Am J Vet Res. 1974 Jan;35(1):107-10.
4
An epornitic of fatal chlamydiosis (ornithosis) in South Carolina turkeys.
J Am Vet Med Assoc. 1975 Jan 15;166(2):175-8.
5
Direct immunofluorescence tests with counterstains for detection of Chlamydia psittaci in infected avian tissues.采用复染剂的直接免疫荧光试验检测感染禽类组织中的鹦鹉热衣原体。
Can J Comp Med. 1979 Apr;43(2):217-22.
6
Studies on defined immunofluorescence in clinical immunopathology. II. Relationship of F/P ratios pf conjugates and staining properties in indirect immunofluorescence.临床免疫病理学中特异性免疫荧光的研究。II. 间接免疫荧光中结合物的F/P比率与染色特性的关系。
Ann N Y Acad Sci. 1975 Jun 30;254:559-72. doi: 10.1111/j.1749-6632.1975.tb29203.x.

用于衣原体抗原荧光抗体偶联物的山羊血清。

Goat serums for fluorescent antibody conjugates to chlamydial antigens.

作者信息

Tessler J

出版信息

Can J Comp Med. 1984 Apr;48(2):228-9.

PMID:6372973
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1236045/
Abstract

Serums from goats hyperimmunized with Chlamydia psittaci consistently produce antichlamydial fluorescent antibody conjugate of high titer. The titer of the fluorescent antibody conjugate prepared from a given serum correlated well with the titer obtained by agar gel precipitin, but not with the complement fixation. The agar gel precipitin test can be used to predict whether a given serum is satisfactory for use in production of a conjugate for direct fluorescent antibody tests. Serums with an agar gel precipitin titer of 1/8 or higher generally produce a usable fluorescent antibody conjugate. Labeling gamma globulins with fluorescein isothiocyanate at a ratio of 1/150 resulted in satisfactory fluorescent antibody conjugates. Cultures of Vero cells infected with chlamydiae were found to be suitable for titration of the fluorescent antibody conjugates.

摘要

用鹦鹉热衣原体超免疫的山羊血清始终能产生高滴度的抗衣原体荧光抗体结合物。从给定血清制备的荧光抗体结合物的滴度与琼脂凝胶沉淀素法测得的滴度相关性良好，但与补体结合试验测得的滴度无关。琼脂凝胶沉淀素试验可用于预测给定血清是否适合用于制备直接荧光抗体试验的结合物。琼脂凝胶沉淀素滴度为1/8或更高的血清通常能产生可用的荧光抗体结合物。以1/150的比例用异硫氰酸荧光素标记γ球蛋白可得到令人满意的荧光抗体结合物。发现感染衣原体的Vero细胞培养物适合用于滴定荧光抗体结合物。