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Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells.

作者信息

Kao F T, Puck T T

出版信息

Proc Natl Acad Sci U S A. 1968 Aug;60(4):1275-81. doi: 10.1073/pnas.60.4.1275.

DOI:10.1073/pnas.60.4.1275
PMID:5244736
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC224914/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/add4/224914/bb9e1af08b5a/pnas00122-0227-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/add4/224914/bb9e1af08b5a/pnas00122-0227-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/add4/224914/bb9e1af08b5a/pnas00122-0227-a.jpg

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Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells.哺乳动物体细胞遗传学,VII. 中国仓鼠细胞中营养缺陷型突变体的诱导与分离
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[Obtaining auxotropic mutants from cultures of Escherichia coli pathogenic serotype O111:B4:H2, treated with N-methyl-N'-nitro-N-nitrosoguanidine].[从经N-甲基-N'-硝基-N-亚硝基胍处理的致病性血清型O111:B4:H2大肠杆菌培养物中获得营养缺陷型突变体]
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Mutation of bacteria at high levels of survival by ethyl methane sulphonate.用甲磺酸乙酯使细菌在高存活水平下发生突变。
Nature. 1959 Dec 5;184:1780-2. doi: 10.1038/1841780a0.
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Control of growth of mammalian cells in culture with folic acid, thymidine, and purines.利用叶酸、胸腺嘧啶核苷和嘌呤控制培养的哺乳动物细胞生长。
J Biol Chem. 1960 Apr;235:1119-23.
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CLONAL GROWTH OF MAMMALIAN CELLS IN A CHEMICALLY DEFINED, SYNTHETIC MEDIUM.哺乳动物细胞在化学成分明确的合成培养基中的克隆生长
Pharmaceuticals (Basel). 2023 Dec 20;17(1):8. doi: 10.3390/ph17010008.
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New high-throughput screening method for Chinese hamster ovary cell lines expressing low reduced monoclonal antibody levels: application of a system controlling the gas phase over cell lysates in miniature bioreactors and facilitating multiple sample setup.用于表达低还原单克隆抗体水平的中国仓鼠卵巢细胞系的新型高通量筛选方法:在微型生物反应器中控制细胞裂解物上方气相并便于进行多样品设置的系统的应用。
Cytotechnology. 2023 Oct;75(5):421-433. doi: 10.1007/s10616-023-00587-x. Epub 2023 Jul 21.
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Effects of the COVID-19 pandemic: new approaches for accelerated delivery of gene to first-in-human CMC data for recombinant proteins.新冠疫情的影响:为重组蛋白的首次人体 CMC 数据的基因加速交付提供新方法。
MAbs. 2023 Jan-Dec;15(1):2220150. doi: 10.1080/19420862.2023.2220150.
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Efficient production of recombinant proteins in suspension CHO cells culture using the Tol2 transposon system coupled with cycloheximide resistance selection.利用 Tol2 转座子系统和环己酰亚胺抗性选择,在悬浮 CHO 细胞培养中高效生产重组蛋白。
Sci Rep. 2023 May 10;13(1):7628. doi: 10.1038/s41598-023-34636-4.
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Influence of Heparan Sulfate Proteoglycans and Factor X on species D Human Adenovirus Uptake and Transduction.硫酸乙酰肝素蛋白聚糖和因子 X 对种 D 人腺病毒摄取和转导的影响。
Viruses. 2022 Dec 24;15(1):55. doi: 10.3390/v15010055.
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10
Genetics of somatic mammalian cells. V. Treatment with 5-bromodeoxyuridine and visible light for isolation of nutritionally deficient mutants.哺乳动物体细胞遗传学。V. 用5-溴脱氧尿苷和可见光处理以分离营养缺陷型突变体。
Proc Natl Acad Sci U S A. 1967 Sep;58(3):1227-34. doi: 10.1073/pnas.58.3.1227.