Denis H, Brachet J
Proc Natl Acad Sci U S A. 1969 Jan;62(1):194-201. doi: 10.1073/pnas.62.1.194.
The technique of molecular hybridization has been used to determine in what proportion the DNA's of paternal and maternal origin are present in the lethal hybrid obtained by fertilizing the eggs of the sea urchin Paracentrotus lividus with the sperm of another sea urchin species, Arbacia lixula. The ability of the hybridization technique to recognize DNA molecules from A. lixula and P. lividus was examined and proved to be satisfactory. Labeled DNA's from A. lixula and P. lividus were found to hybridize respectively ten and seven times as well with DNA of their own species as with DNA of the other species. Labeled DNA from arrested hybrid embryos hybridized 1.7 times to twice as well with P. lividus as with A. lixula DNA. We conclude that the interspecific hybrid contains about 2.5 times as much DNA of maternal origin as DNA of paternal origin. The lower content of A. lixula DNA probably results from a partial elimination of the paternal chromosomes during the late cleavage stages.
分子杂交技术已被用于确定,在用另一种海胆物种——光棘球海胆(Arbacia lixula)的精子使地中海海胆(Paracentrotus lividus)的卵子受精所获得的致死杂交体中,父本和母本来源的DNA各占多大比例。对杂交技术识别来自光棘球海胆和地中海海胆的DNA分子的能力进行了检测,结果证明令人满意。发现来自光棘球海胆和地中海海胆的标记DNA与自身物种的DNA杂交的程度分别是与另一物种DNA杂交程度的10倍和7倍。来自停滞发育的杂交胚胎的标记DNA与地中海海胆DNA杂交的程度是与光棘球海胆DNA杂交程度的1.7倍至两倍。我们得出结论,种间杂交体中母本来源的DNA含量大约是父本来源DNA含量的2.5倍。光棘球海胆DNA含量较低可能是由于在后期卵裂阶段父本染色体部分消除所致。