将兔血红蛋白信使核糖核酸显微注射到两栖类卵母细胞和胚胎中。
Microinjection of rabbit hemoglobin messenger RNA into amphibian oocytes and embryos.
作者信息
Brachet J, Huez G, Hubert E
出版信息
Proc Natl Acad Sci U S A. 1973 Feb;70(2):543-7. doi: 10.1073/pnas.70.2.543.
After microinjection of hemoglobin mRNA from rabbit reticulocytes (9S mRNA) into Xenopus laevis oocytes, the cytological structure remains normal. The peroxidase reaction does not show an accumulation of synthesized hemoglobin in the germinal vesicle. Fertilized Pleurodeles Waltlii eggs synthesize rabbit hemoglobin after injection of 9S mRNA; hemoglobin synthesis decreases as development progresses. Micromeres and macromeres synthesize equal amounts of hemoglobin, despite the fact that the micromeres contain many more ribosomes. Descendants of injected blastomeres divide more slowly and do not differentiate; they ultimately undergo cytolysis.
将兔网织红细胞的血红蛋白mRNA（9S mRNA）显微注射到非洲爪蟾卵母细胞后，其细胞学结构保持正常。过氧化物酶反应未显示出生殖泡中有合成的血红蛋白积累。注射9S mRNA后，受精的欧洲螈卵合成兔血红蛋白；随着发育进程，血红蛋白合成减少。尽管小分裂球含有更多核糖体，但小分裂球和大分裂球合成等量的血红蛋白。注射的卵裂球后代分裂更慢且不分化；它们最终会发生细胞溶解。
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