30S核糖体亚基上存在一个功能性苯丙氨酰-tRNA结合位点的证据。
Evidence for one functional phenylalanyl-tRNA binding site on the 30S ribosomal subunit.
作者信息
Igarashi K, Kaji A
出版信息
Proc Natl Acad Sci U S A. 1969 Feb;62(2):498-505. doi: 10.1073/pnas.62.2.498.
Abstract
NH(2) terminal analysis of polyphenylalanine formed from 30S subunit-bound (14)C-phenylalanyl-tRNA suggests that there is only one site in a 30S subunit for specific binding of phenylalanyl-tRNA. Assuming that no movement of the bound phenylalanyl-tRNA takes place during association of 30S subunits with 50S ribosomal subunits, the binding site of the 30S ribosomal subunit corresponds to site 2 (-NH(2) side with respect to the growing polypeptide chain) of the two binding sites of 70S ribosomes.
摘要
对由与30S亚基结合的（14）C-苯丙氨酰-tRNA形成的聚苯丙氨酸进行的N（2）末端分析表明，30S亚基中只有一个位点可特异性结合苯丙氨酰-tRNA。假设在30S亚基与50S核糖体亚基结合过程中，结合的苯丙氨酰-tRNA不发生移动，那么30S核糖体亚基的结合位点对应于70S核糖体两个结合位点中的位点2（相对于正在生长的多肽链的-NH（2）侧）。
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