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30S核糖体亚基上存在一个功能性苯丙氨酰-tRNA结合位点的证据。

Evidence for one functional phenylalanyl-tRNA binding site on the 30S ribosomal subunit.

作者信息

Igarashi K, Kaji A

出版信息

Proc Natl Acad Sci U S A. 1969 Feb;62(2):498-505. doi: 10.1073/pnas.62.2.498.

DOI:10.1073/pnas.62.2.498
PMID:5256228
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC277826/
Abstract

NH(2) terminal analysis of polyphenylalanine formed from 30S subunit-bound (14)C-phenylalanyl-tRNA suggests that there is only one site in a 30S subunit for specific binding of phenylalanyl-tRNA. Assuming that no movement of the bound phenylalanyl-tRNA takes place during association of 30S subunits with 50S ribosomal subunits, the binding site of the 30S ribosomal subunit corresponds to site 2 (-NH(2) side with respect to the growing polypeptide chain) of the two binding sites of 70S ribosomes.

摘要

对由与30S亚基结合的（14）C-苯丙氨酰-tRNA形成的聚苯丙氨酸进行的N（2）末端分析表明，30S亚基中只有一个位点可特异性结合苯丙氨酰-tRNA。假设在30S亚基与50S核糖体亚基结合过程中，结合的苯丙氨酰-tRNA不发生移动，那么30S核糖体亚基的结合位点对应于70S核糖体两个结合位点中的位点2（相对于正在生长的多肽链的-NH（2）侧）。

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Proc Natl Acad Sci U S A. 1969 Feb;62(2):498-505. doi: 10.1073/pnas.62.2.498.
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引用本文的文献

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Plant Physiol. 1971 Nov;48(5):547-52. doi: 10.1104/pp.48.5.547.

本文引用的文献

1
THE EFFECT OF POLYAMINES AND OF POLY U SIZE ON PHENYLALANINE INCORPORATION.多胺及聚尿苷酸大小对苯丙氨酸掺入的影响
Proc Natl Acad Sci U S A. 1962 Dec;48(12):2171-8. doi: 10.1073/pnas.48.12.2171.
2
RNA CODEWORDS AND PROTEIN SYNTHESIS. THE EFFECT OF TRINUCLEOTIDES UPON THE BINDING OF SRNA TO RIBOSOMES.RNA密码子与蛋白质合成。三核苷酸对可溶性核糖核酸（sRNA）与核糖体结合的影响。
Science. 1964 Sep 25;145(3639):1399-407. doi: 10.1126/science.145.3639.1399.
3
Amino acid transfer from aminoacyl-ribonucleic acids to protein on ribosomes of Escherichia coli.氨基酸在大肠杆菌核糖体上从氨酰核糖核酸转移至蛋白质的过程。
Proc Natl Acad Sci U S A. 1961 Apr 15;47(4):497-504. doi: 10.1073/pnas.47.4.497.
4
Regulatory mechanisms and protein synthesis. X. Codon recognition on 30 S ribosomes.调控机制与蛋白质合成。十、30 S核糖体上的密码子识别
J Mol Biol. 1966 Oct 28;21(1):145-71. doi: 10.1016/0022-2836(66)90085-4.
5
Binding of specific sRNA to 30S ribosomal subunits: effect of 50S ribosomal subunits.特定小RNA与30S核糖体亚基的结合：50S核糖体亚基的作用
Proc Natl Acad Sci U S A. 1966 Jun;55(6):1483-90. doi: 10.1073/pnas.55.6.1483.
6
Binding of specific soluble ribonucleic acid to ribosomes. Binding of soluble ribonucleic acid to the template-30 S subunits complex.特定可溶性核糖核酸与核糖体的结合。可溶性核糖核酸与模板 - 30S亚基复合物的结合。
J Biol Chem. 1966 Mar 25;241(6):1251-6.
7
Binding of specific sRNA to 30S subunits--comparison with the binding to 70S ribosomes.特定小RNA与30S亚基的结合——与70S核糖体结合的比较。
Biochem Biophys Res Commun. 1965 Oct 26;21(2):187-93. doi: 10.1016/0006-291x(65)90107-5.
8
Factor- and guanosine 5'-triphosphate-dependent release of deacylated transfer RNA from 70S ribosomes.70S核糖体上依赖因子和鸟苷5'-三磷酸的去酰化转运RNA的释放
Proc Natl Acad Sci U S A. 1968 Dec;61(4):1399-405. doi: 10.1073/pnas.61.4.1399.
9
Translation of the genetic message. VII. Role of initiation factors in formation of the chain initiation complex with Escherichia coli ribosomes.遗传信息的翻译。VII. 起始因子在与大肠杆菌核糖体形成链起始复合物中的作用。
Arch Biochem Biophys. 1968 May;125(2):542-7. doi: 10.1016/0003-9861(68)90612-7.
10
Role of protein factor in the functional binding of ribosomes to natural messenger RNA.蛋白质因子在核糖体与天然信使核糖核酸功能结合中的作用。
J Mol Biol. 1968 Apr 14;33(1):231-49. doi: 10.1016/0022-2836(68)90291-x.