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关于原核生物蛋白质合成中起始tRNA甲硫氨酸甲酰化的理解。I. 30S和70S核糖体 - tRNA复合物的体外研究。

Toward an understanding of the formylation of initiator tRNA methionine in prokaryotic protein synthesis. I. In vitro studies of the 30S and 70S ribosomal-tRNA complex.

作者信息

Petersen H U, Danchin A, Grunberg-Manago M

出版信息

Biochemistry. 1976 Apr 6;15(7):1357-62. doi: 10.1021/bi00652a001.

DOI:10.1021/bi00652a001
PMID:769821
Abstract

Formation of the 30S-tRNA initiation complex of Escherichia coli with nonformylated initiator tRNA is stimulated by all three initiation factors and is messenger dependent, whereas the complex formation involving the 70S ribosomes is strongly inhibited by initiation factors when the nonformylated species is used. When the 30S-Met-tRNAfMet complex is first formed and the 50S ribosomal subunit added subsequently, there is no significant inhibition by initiation factors and the nonformylated initiator tRNA is puromycin reactive. This leads to the conclusion that the formylation of the methionyl initator tRNA is only obligatory when polypeptide synthesis is initiated by nondissociated 70S ribosomes.

摘要

大肠杆菌30S-tRNA起始复合物与非甲酰化起始tRNA的形成受到所有三种起始因子的刺激，且依赖于信使核糖核酸，而当使用非甲酰化种类时，涉及70S核糖体的复合物形成受到起始因子的强烈抑制。当首先形成30S-Met-tRNAfMet复合物，随后添加50S核糖体亚基时，起始因子没有明显抑制作用，且非甲酰化起始tRNA对嘌呤霉素有反应。这得出结论，只有当多肽合成由未解离的70S核糖体起始时，甲硫氨酰起始tRNA的甲酰化才是必需的。

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1
Toward an understanding of the formylation of initiator tRNA methionine in prokaryotic protein synthesis. I. In vitro studies of the 30S and 70S ribosomal-tRNA complex.关于原核生物蛋白质合成中起始tRNA甲硫氨酸甲酰化的理解。I. 30S和70S核糖体 - tRNA复合物的体外研究。
Biochemistry. 1976 Apr 6;15(7):1357-62. doi: 10.1021/bi00652a001.
2
Toward an understanding of the formylation of initiator tRNA methionine in prokaryotic protein synthesis. II. A two-state model for the 70S ribosome.关于原核生物蛋白质合成中起始tRNA甲硫氨酸甲酰化的理解。II. 70S核糖体的双态模型。
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Proceedings: Role of reversible association of ribosomal subunits, of initiation factor IF3, and of formylation of Met-tRNAfMet in protein synthesis initiation.论文集：核糖体亚基的可逆结合、起始因子IF3以及甲硫氨酰 - tRNAfMet的甲酰化在蛋白质合成起始中的作用
J Biochem. 1976 Apr;79(4):31P-32P.
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Proc Natl Acad Sci U S A. 1972 May;69(5):1234-8. doi: 10.1073/pnas.69.5.1234.
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A complex between initiation factor IF2, guanosine triphosphate, and fMet-tRNA: an intermediate in initiation complex formation.起始因子IF2、鸟苷三磷酸和甲酰甲硫氨酸转运核糖核酸之间的复合物：起始复合物形成过程中的一个中间体。
Proc Natl Acad Sci U S A. 1971 Dec;68(12):3122-6. doi: 10.1073/pnas.68.12.3122.
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Initiation factor 2-dependent ribosomal binding of N-formylmethionyl-transfer RNA without added guanosine triphosphate.在不添加鸟苷三磷酸的情况下，起始因子2依赖的N-甲酰甲硫氨酰-转运RNA的核糖体结合。
Proc Natl Acad Sci U S A. 1972 Oct;69(10):2770-3. doi: 10.1073/pnas.69.10.2770.

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