一种在激素与基因组相互作用中起中介作用的蛋白质。
A protein intermediary in the interaction of a hormone with the genome.
作者信息
Matthysse A G, Phillips C
出版信息
Proc Natl Acad Sci U S A. 1969 Jul;63(3):897-903. doi: 10.1073/pnas.63.3.897.
Abstract
The increased rate of RNA synthesis by target cells caused by the plant hormone auxin has been studied as an example of hormonal regulation of transcription. The hormone does not interact directly with chromatin but requires a protein mediator. In the presence of this mediator, auxin increases the rate of RNA synthesis both by isolated plant nuclei and by isolated chromatin. This increased rate of RNA synthesis occurs even in the presence of saturating amounts of RNA polymerase. The hormone and protein do not affect the rate of RNA synthesis if pure DNA is used as the template. The results suggest that auxin plus the protein increase the rate of RNA synthesis by making an increased portion of the genome available for transcription.
摘要
植物激素生长素引起靶细胞RNA合成速率增加，这一现象已作为转录激素调节的一个例子进行了研究。该激素不直接与染色质相互作用，而是需要一种蛋白质介导物。在这种介导物存在的情况下，生长素可提高分离的植物细胞核和分离的染色质的RNA合成速率。即使存在饱和量的RNA聚合酶，RNA合成速率仍会增加。如果使用纯DNA作为模板，该激素和蛋白质不会影响RNA合成速率。结果表明，生长素与该蛋白质一起通过使基因组中更多部分可用于转录来提高RNA合成速率。
相似文献
1
A protein intermediary in the interaction of a hormone with the genome.一种在激素与基因组相互作用中起中介作用的蛋白质。
Proc Natl Acad Sci U S A. 1969 Jul;63(3):897-903. doi: 10.1073/pnas.63.3.897.
2
Effect of growth hormone on ribonucleic acid metabolism. The template activity of the chromatin and molecular species of ribonucleic acid synthesized after treatment with the hormone.生长激素对核糖核酸代谢的影响。激素处理后染色质的模板活性及合成的核糖核酸分子种类。
Biochem J. 1968 Dec;110(3):401-6. doi: 10.1042/bj1100401.
3
Enhancement of soybean RNA polymerase I by auxin.生长素对大豆RNA聚合酶I的增强作用。
Proc Natl Acad Sci U S A. 1975 Jan;72(1):69-72. doi: 10.1073/pnas.72.1.69.
4
Growth hormone and drug metabolism. Acute effects on nuclear ribonucleic acid polymerase activity and chromatin.生长激素与药物代谢。对核糖核酸聚合酶活性和染色质的急性影响。
Biochem J. 1976 Feb 15;154(2):439-48. doi: 10.1042/bj1540439.
5
Hormonal regulation of leaf senescence.叶片衰老的激素调节
Symp Soc Exp Biol. 1967;21:305-21.
6
Dinucleotide priming of RNA synthesis.
Cytogenet Cell Genet. 1980;26(2-4):211-22. doi: 10.1159/000131442.
7
Noncoding transcription by alternative RNA polymerases dynamically regulates an auxin-driven chromatin loop.非编码转录由替代 RNA 聚合酶动态调控生长素驱动的染色质环。
Mol Cell. 2014 Aug 7;55(3):383-96. doi: 10.1016/j.molcel.2014.06.011. Epub 2014 Jul 10.
8
Auxin-regulated gene expression.生长素调节的基因表达。
Philos Trans R Soc Lond B Biol Sci. 1986 Nov 17;314(1166):427-40. doi: 10.1098/rstb.1986.0063.
9
Histones, chromatin structure and RNA synthesis.
Nat New Biol. 1972 May 17;237(72):87-8. doi: 10.1038/newbio237087a0.
10
Auxin receptors and plant development: a new signaling paradigm.生长素受体与植物发育：一种新的信号传导模式
Annu Rev Cell Dev Biol. 2008;24:55-80. doi: 10.1146/annurev.cellbio.23.090506.123214.
引用本文的文献
1
Effects of abscisic acid on the soluble RNA polymerase activity in maize coleoptiles.脱落酸对玉米幼茎可溶性 RNA 聚合酶活性的影响。
Planta. 1972 Mar;103(1):11-7. doi: 10.1007/BF00394601.
2
The effect of auxin on the incorporation of [(3)H]thymidine into the DNA of pea epicotyls.生长素对豌豆上胚轴 DNA 掺入 [(3)H]胸腺嘧啶核苷的影响。
Planta. 1974 Mar;116(1):65-72. doi: 10.1007/BF00390204.
3
Indole-acetic acid binding proteins in soybean cotyledon.大豆子叶中的吲哚乙酸结合蛋白。
Planta. 1976 Jan;131(3):223-8. doi: 10.1007/BF00385419.
4
Gibberellic acid-Binding proteins from pea stems.豌豆茎中的赤霉素结合蛋白。
Planta. 1976 Jan;131(2):125-8. doi: 10.1007/BF00389982.
5
Ribonucleic acid synthesis by chromatin isolated from Phaseolus aureus Roxb. : The effect of endogenous ribonuclease.从 Phaseolus aureus Roxb. 分离的染色质的核糖核酸合成：内源性核糖核酸酶的影响。
Planta. 1977 Jan;137(2):153-7. doi: 10.1007/BF00387552.
6
Effect of 2,4-dichlorophenoxyacetic Acid on glucosylation of scopoletin to scopolin in tobacco tissue culture.2,4-二氯苯氧乙酸对烟草组织培养中紫菀酮糖基化生成紫菀酮的影响。
Plant Physiol. 1982 Apr;69(4):810-3. doi: 10.1104/pp.69.4.810.
7
Isolation and translation of plant messenger RNA.植物信使 RNA 的分离与翻译。
Plant Physiol. 1975 Jul;56(1):88-92. doi: 10.1104/pp.56.1.88.
8
Photoaffinity-labeled Auxins: Synthesis and Biological Activity.光亲和标记生长素：合成与生物活性。
Plant Physiol. 1975 Jun;55(6):1057-61. doi: 10.1104/pp.55.6.1057.
9
Auxin and ribonucleic Acid synthesis in pea stem tissue as studied by deoxyribonucleic Acid-ribonucleic Acid hybridization.通过脱氧核糖核酸-核糖核酸杂交研究豌豆茎组织中的生长素与核糖核酸合成
Plant Physiol. 1971 Dec;48(6):663-70. doi: 10.1104/pp.48.6.663.
10
Inhibition of Auxin-induced Deoxyribonucleic Acid Synthesis and Chromatin Activity by 5-Fluorodeoxyuridine in Soybean Hypocotyl.5-氟脱氧尿苷对大豆下胚轴中生长素诱导的脱氧核糖核酸合成和染色质活性的抑制作用。
Plant Physiol. 1971 May;47(5):606-8. doi: 10.1104/pp.47.5.606.
本文引用的文献
1
DIORTHO SUBSTITUTED PHENOXYACETIC ACIDS AS ANTIAUXINS.作为抗生长素的二邻位取代苯氧基乙酸类化合物
Plant Physiol. 1952 Oct;27(4):834-8. doi: 10.1104/pp.27.4.834.
2
Histone, a suppressor of chromosomal RNA synthesis.组蛋白，一种染色体RNA合成的抑制剂。
Proc Natl Acad Sci U S A. 1962 Jul 15;48(7):1216-22. doi: 10.1073/pnas.48.7.1216.
3
Deoxyribo ucleic acid-directed synthesis of ribonucleic acid by an enzyme from Escherichia coli.大肠杆菌中一种酶催化的脱氧核糖核酸指导的核糖核酸合成。
Proc Natl Acad Sci U S A. 1962 Jan 15;48(1):81-94. doi: 10.1073/pnas.48.1.81.
4
Template properties of liver chromatin.肝脏染色质的模板特性
J Mol Biol. 1966 Jan;15(1):160-74. doi: 10.1016/s0022-2836(66)80218-8.
5
Semi-conservative replication of DNA in a higher plant cell.高等植物细胞中DNA的半保留复制。
Exp Cell Res. 1965 Aug;39(1):33-9. doi: 10.1016/0014-4827(65)90004-2.
6
Chromosomal ribonucleic acid of rat ascites cells.大鼠腹水细胞的染色体核糖核酸
Biochemistry. 1969 Apr;8(4):1524-34. doi: 10.1021/bi00832a031.
7
Enhancement of RNA synthesis in isolated pea nuclei by gibberellic acid.赤霉素对离体豌豆细胞核中RNA合成的促进作用。
Proc Natl Acad Sci U S A. 1968 Jan;59(1):269-76. doi: 10.1073/pnas.59.1.269.
8
Template capacity of uterine chromatin: control by estradiol.子宫染色质的模板容量：受雌二醇调控。
Proc Natl Acad Sci U S A. 1966 Oct;56(4):1298-302. doi: 10.1073/pnas.56.4.1298.
9
A receptor molecule for estrogens: isolation from the rat uterus and preliminary characterization.一种雌激素受体分子：从大鼠子宫中分离及初步特性鉴定。
Proc Natl Acad Sci U S A. 1966 Jun;55(6):1574-81. doi: 10.1073/pnas.55.6.1574.
10
Hormonal regulation of growth and protein synthesis.生长与蛋白质合成的激素调节。
Nature. 1968 Jul 27;219(5152):331-7. doi: 10.1038/219331a0.
Zotero 插件