二硫苏糖醇对亲和标记试剂与乙酰胆碱酯酶及电鳗电板可兴奋膜相互作用的影响比较。
Compared effects of dithiotreitol on the interaction of an affinity-labeling reagent with acetylcholinesterase and the excitable membrane of the electroplax.
作者信息
Podleski T, Meunier J C, Changeux J P
出版信息
Proc Natl Acad Sci U S A. 1969 Aug;63(4):1239-46. doi: 10.1073/pnas.63.4.1239.
Abstract
p-(trimethyl ammonium) benzene diazonium difluoroborate (TDF), an affinity-labeling reagent of the acetylcholine receptor site(s), which in the normal cell acts as an irreversible inhibitor becomes a reversible activator after in vivo exposure of the electroplax to dithiothreitol (DTT), a disulfide bond reducing agent. After in vitro exposure of acetylcholinesterase to DTT, TDF becomes a reversible competitive inhibitor of the enzyme, using indophenyl acetate as the substrate. Both acetylcholinesterase and the macromolecular receptor of acetylcholine thus contain disulfide bonds. Additional experiments with DTT suggest that there might exist several different classes of receptor sites for cholinergic agents in the excitable membrane of the electroplax.
摘要
对-（三甲铵基）苯重氮二氟硼酸盐（TDF），一种乙酰胆碱受体位点的亲和标记试剂，在正常细胞中作为不可逆抑制剂起作用，在电鳐体内暴露于二硫苏糖醇（DTT，一种二硫键还原剂）后成为可逆激活剂。在体外将乙酰胆碱酯酶暴露于DTT后，以醋酸吲哚酚为底物时，TDF成为该酶的可逆竞争性抑制剂。因此，乙酰胆碱酯酶和乙酰胆碱的大分子受体都含有二硫键。用DTT进行的其他实验表明，在电鳐可兴奋膜中可能存在几种不同类型的胆碱能剂受体位点。
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本文引用的文献
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On the cooperativity of biological membranes.论生物膜的协同性。
Proc Natl Acad Sci U S A. 1967 Feb;57(2):335-41. doi: 10.1073/pnas.57.2.335.
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On the irreversible binding of p-(trimethylammonium) benzenediazonium fluoroborate (TDF) to acetylcholinesterase from electrogenic tissue.关于对-(三甲铵基)苯重氮氟硼酸盐(TDF)与电生组织中的乙酰胆碱酯酶的不可逆结合
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Biochim Biophys Acta. 1967 Jul 11;139(2):358-62. doi: 10.1016/0005-2744(67)90039-3.
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On the nature of allosteric transitions: implications of non-exclusive ligand binding.关于别构转变的本质：非排他性配体结合的影响
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