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1
On Some Structural Analogies between Acetylcholinesterase and the Macromolecular Receptor of Acetylcholine.乙酰胆碱酯酶与乙酰胆碱大分子受体的某些结构类似性。
J Gen Physiol. 1969 Jul 1;54(1):225-44. doi: 10.1085/jgp.54.1.225.
2
Compared effects of dithiotreitol on the interaction of an affinity-labeling reagent with acetylcholinesterase and the excitable membrane of the electroplax.二硫苏糖醇对亲和标记试剂与乙酰胆碱酯酶及电鳗电板可兴奋膜相互作用的影响比较。
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引用本文的文献

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J Membr Biol. 1972 Dec;9(1):155-76. doi: 10.1007/BF01868050.
2
In vitro excitation of purified membrane fragments by cholinergic agonists : III. Comparison of the dose-response curves to decamethonium with the corresponding binding curves of decamethonium to the cholinergic receptor.在体外用胆堿能激动剂刺激纯化的膜片段：III. 脒甲酰胆碱的剂量-反应曲线与脒甲酰胆碱对胆堿能受体的相应结合曲线的比较。
J Membr Biol. 1971 Mar;6(1):58-80. doi: 10.1007/BF01874114.
3
In Vitro excitation of purified membrane fragments by cholinergic agonists : II. The permeability change caused by cholinergic agonists.在体纯化膜片段的胆碱能激动剂刺激：II. 胆碱能激动剂引起的通透性变化。
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pH optimum for the rate of acetylcholine action on neurons.乙酰胆碱作用于神经元的速率的最适pH值。
Agents Actions. 1971 Nov;2(3):131-7. doi: 10.1007/BF01966751.
6
The effects of reducing and alkylating agents on the acetylcholine receptor activity of frog sartorius muscle.还原和烷基化试剂对青蛙缝匠肌乙酰胆碱受体活性的影响。
J Membr Biol. 1973 Feb 27;11(3):217-26. doi: 10.1007/BF01869823.
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8
An ESR study of the postsynatpic membrane acetylcholinesterase of Torpedo marmorata electric organ.斑纹电鳐电器官突触后膜乙酰胆碱酯酶的电子自旋共振研究。
Mol Cell Biochem. 1976 Dec 10;13(3):137-9. doi: 10.1007/BF01731775.

乙酰胆碱酯酶与乙酰胆碱大分子受体的某些结构类似性。

On Some Structural Analogies between Acetylcholinesterase and the Macromolecular Receptor of Acetylcholine.

机构信息

Service de Biochimie Cellulaire, Institut Pasteur, Paris, France.

出版信息

J Gen Physiol. 1969 Jul 1;54(1):225-44. doi: 10.1085/jgp.54.1.225.

DOI:10.1085/jgp.54.1.225
PMID:19873643
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2225907/
Abstract

Several properties of the enzyme acetylcholinesterase (AChE) isolated in vitro are compared with those of the membrane receptor(s) of acetylcholine expressed by the in vivo electrical response of the electroplax membrane. AChE strongly binds in vitro effectors of the electroplax: agonists e.g., decamethonium or antagonists, e.g., d-tubocurarine and flaxedil. It also reacts covalently with an affinity labeling reagent of the acetylcholine receptor site(s) in vivo (TDF). Two classes of sites on AChE molecule account for the binding of these quaternary nitrogen containing compounds: (1) the anionic site of the active center and (2) noncatalytic "peripheral anionic centers" located outside the active center. A disulfide bond breaking agent, dithiothreitol (DTT) alters in a parallel manner the reaction of AChE and the excitable membrane of the electroplax to TDF. The irreversibility of TDF action is lost in both cases, after exposure to DTT. Both AChE and the acetylcholine receptor thus contain disulfide bonds-they are closely related but not necessarily identical proteins.

摘要

将离体分离的乙酰胆碱酯酶（AChE）的几种性质与电鳐膜体内电反应表达的乙酰胆碱膜受体的性质进行比较。AChE 强烈结合电鳐的体外效应物：激动剂如十甲季铵或拮抗剂，如 d-筒箭毒碱和花椒毒素。它还与体内乙酰胆碱受体部位的亲和标记试剂 TDF 发生共价反应。AChE 分子上的两类结合部位负责结合这些含季铵氮的化合物：（1）活性中心的阴离子部位和（2）位于活性中心外的非催化“外周阴离子中心”。二硫键断裂剂二硫苏糖醇（DTT）以平行的方式改变 AChE 和电鳐可兴奋膜对 TDF 的反应。在这两种情况下，暴露于 DTT 后，TDF 的作用都失去了不可逆性。因此，AChE 和乙酰胆碱受体都含有二硫键——它们是密切相关但不一定相同的蛋白质。