膜结合核糖体和游离核糖体在体外合成不同类别的特定蛋白质。
In vitro synthesis of different categories of specific protein by membrane-bound and free ribosomes.
作者信息
Ganoza M C, Williams C A
出版信息
Proc Natl Acad Sci U S A. 1969 Aug;63(4):1370-6. doi: 10.1073/pnas.63.4.1370.
Abstract
The free and membrane-bound ribosomes of hepatic cells were isolated and used to program protein synthesis in cell-free extracts. Immuno-electrophoretic and radioautographic analysis of the products showed that the membrane-bound ribosomes synthesized serum proteins, whereas ribosomes free of endoplasmic reticulum synthesized specific nonserum liver proteins. After synthesis, the serum proteins remained associated with membrane vesicles, whereas the other liver proteins were found free of cytoplasmic organelles.
摘要
分离出肝细胞的游离核糖体和膜结合核糖体，并用于在无细胞提取物中指导蛋白质合成。对产物进行免疫电泳和放射自显影分析表明，膜结合核糖体合成血清蛋白，而游离于内质网的核糖体合成特定的非血清肝蛋白。合成后，血清蛋白仍与膜泡结合，而其他肝蛋白则存在于无细胞质细胞器的状态。
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