免疫球蛋白的合成与分泌。V. 亮氨酸和氨基葡萄糖掺入游离及结合多核糖体上的免疫球蛋白的情况。
Immunoglobulin synthesis and secretion. V. Incorporation of leucine and glucosamine into immunoglobulin on free and bound polyribosomes.
作者信息
Sherr C J, Uhr J W
出版信息
Proc Natl Acad Sci U S A. 1970 Aug;66(4):1183-9. doi: 10.1073/pnas.66.4.1183.
Abstract
Mouse myeloma cells pulse-labeled in vitro with (3)H-leucine or (3)H-glucosamine were fractionated on sucrose gradients, and membrane-bound and free polyribosomes were separated. Nascent polypeptides released from polyribosomes were precipitated with antiserum specific for mouse immunoglobulin. The results indicate that immunoglobulin is synthesized preferentially by bound polyribosomes.
摘要
用³H-亮氨酸或³H-葡萄糖胺在体外进行脉冲标记的小鼠骨髓瘤细胞，在蔗糖梯度上进行分级分离，从而分离出膜结合型和游离型多核糖体。从多核糖体释放的新生多肽用针对小鼠免疫球蛋白的抗血清沉淀。结果表明，免疫球蛋白优先由结合型多核糖体合成。
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