大肠杆菌噬菌体R17 RNA中尿苷的光水合作用。II. 紫外线灭活与尿苷光水合作用之间的关系。
Photohydration of uridine in the RNA of coliphage R17. II. The relationship between ultraviolet inactivation and uridine photohydration.
作者信息
Remsen J F, Miller N, Cerutti P A
出版信息
Proc Natl Acad Sci U S A. 1970 Feb;65(2):460-6. doi: 10.1073/pnas.65.2.460.
Abstract
Uridine photohydrates (6-hydroxy-5,6-dihydrouridine) are the major products formed upon irradiation of the RNA bacteriophage R17 with ultraviolet light at 280 mmu, while cyclobutane-type pyrimidine dimerization does not occur to an appreciable extent. The suppression of dimerization may indicate that the RNA inside the phage is held in a rigid conformation in close contact with the phage protein. A value of 0.94 (+/-0.10) uridine photohydrates per biological hit is obtained from a correlation of the fraction of phage surviving with the number of photohydrates formed as a function of ultraviolet dose. It is concluded that uridine photohydrates represent a major part of the lethal damage caused by ultraviolet light in bacteriophage R17. An efficient system for dark repair of uridine photohydration seems to be lacking in E. coli.
摘要
尿苷光水合物（6-羟基-5,6-二氢尿苷）是RNA噬菌体R17在280毫微米紫外线下照射时形成的主要产物，而环丁烷型嘧啶二聚化在很大程度上不会发生。二聚化的抑制可能表明噬菌体内部的RNA以与噬菌体蛋白质紧密接触的刚性构象存在。从存活噬菌体的比例与作为紫外线剂量函数形成的光水合物数量的相关性中得出，每个生物学命中的尿苷光水合物值为0.94（±0.10）。可以得出结论，尿苷光水合物是噬菌体R17中紫外线造成的致死损伤的主要部分。大肠杆菌似乎缺乏一种有效的尿苷光水合暗修复系统。
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