前叉合成：一种DNA复制模型。
Pre-fork synthesis: a model for DNA replication.
作者信息
Haskell E H, Daverin C I
出版信息
Proc Natl Acad Sci U S A. 1969 Nov;64(3):1065-71. doi: 10.1073/pnas.64.3.1065.
Abstract
A model of DNA replication is presented in which DNA synthesis is continuously initiated from parental strand nicks and occurs, with conservation of helix winding number, ahead of the so-called replicating fork. The fork in this model is the locus of unwinding of already replicated, but presumably unstable, DNA. The model, involving Okazaki's notion of multiple initiation, is based upon the properties of Kornberg's DNA polymerase and accounts for the presence of single-stranded nascent DNA fragments in cell lysates. In addition to acting as sites of initiation, the parental strand nicks are implicated as sites of free rotation allowing unwinding of the replicated DNA.
摘要
本文提出了一种DNA复制模型，其中DNA合成从亲代链切口处持续起始，并在所谓的复制叉之前进行，同时保持螺旋缠绕数不变。该模型中的复制叉是已复制但可能不稳定的DNA解旋的位点。这个涉及冈崎多重起始概念的模型基于科恩伯格DNA聚合酶的特性，并解释了细胞裂解物中存在单链新生DNA片段的现象。亲代链切口除了作为起始位点外，还被认为是自由旋转的位点，允许复制的DNA解旋。
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