Austen D E
Br J Haematol. 1979 Dec;43(4):669-74. doi: 10.1111/j.1365-2141.1979.tb03800.x.
A new method of factor VIII purification has been devised which involves chromatographic separation on aminohexyl-substituted agarose. Relatively large volumes of starting material can be processed compared to the volume of agarose employed and satisfactory yields are obtained. Factor-VIII-clotting activity is separated from the other related substances and resultant products appear to be stable. While separation of clotting activity occurs with relative ease, the antigen and ristocetin co-factor are hardly segregated, if at all. This provides a little more information about the interrelation of these substances. Results suggest that ion-exchange is involved in the mechanism of separation but additional hydrophobic or steric effects cannot be ruled out.
已设计出一种新的因子 VIII 纯化方法，该方法涉及在氨基己基取代的琼脂糖上进行色谱分离。与所用琼脂糖的体积相比，可以处理相对大量的起始材料，并获得令人满意的产量。因子 VIII 凝血活性与其他相关物质分离，所得产品似乎很稳定。虽然凝血活性的分离相对容易，但抗原和瑞斯托霉素辅因子几乎没有分离（如果有分离的话）。这提供了关于这些物质相互关系的更多信息。结果表明，离子交换参与了分离机制，但不能排除额外的疏水或空间效应。
