一种在琼脂糖凝胶中检测与因子 VIII 相关抗原相关的因子 VIII 凝血活性的方法。

A method for detecting factor-VIII clotting activity associated with factor VIII-related antigen in agarose gels.

作者信息

Bird P, Rizza C R

出版信息

Br J Haematol. 1975 Sep;31(1):5-12. doi: 10.1111/j.1365-2141.1975.tb00826.x.

DOI:10.1111/j.1365-2141.1975.tb00826.x

Abstract

A method for detecting factor-VIII clotting activity in agarose is described. It is based on factor VIII promoting coagulation in a mixture of haemophilic plasma in agarose which is detected by a change in opacity. When this test was used to detect factor-VIII clotting activity in a one-dimensional Laurell electroimmunoassay for factor VIII-related antigen all the factor-VIII activity was found in the same position as the factor VIII-related antigen immunoprecipitate. Factor-VIII clotting activity did not appear to be simply trapped in this immunoprecipitate and therefore it has been concluded that the molecule containing factor-VIII clotting activity carries factor VIII-related antigen determinants.

摘要

本文描述了一种在琼脂糖中检测因子VIII凝血活性的方法。该方法基于因子VIII在琼脂糖中的血友病血浆混合物中促进凝血，通过不透明度的变化来检测。当该试验用于在一维Laurell电免疫分析中检测与因子VIII相关抗原的因子VIII凝血活性时，所有因子VIII活性都位于与因子VIII相关抗原免疫沉淀物相同的位置。因子VIII凝血活性似乎并非简单地被困在该免疫沉淀物中，因此得出结论，含有因子VIII凝血活性的分子携带因子VIII相关抗原决定簇。