制备技术对人型支原体大体形态的影响。
Effect of preparatory techniques on the gross morphology of Mycoplasma hominis.
作者信息
Robertson J, Gomersall M, Gill P
出版信息
J Bacteriol. 1975 Nov;124(2):1019-22. doi: 10.1128/jb.124.2.1019-1022.1975.
Abstract
A method is presented whereby cells of Mycoplasma hominis can be prepared with minimal distortion for electron microscopy. After the addition of glutaraldehyde to broth cultures, incubation is continued for 1 h. The cells are then collected by centrifugation, washed in distilled water, and used for negative-contrast preparations.
摘要
本文介绍了一种方法，通过该方法可以制备人型支原体细胞，使其在电子显微镜下的变形最小。向肉汤培养物中加入戊二醛后，继续孵育1小时。然后通过离心收集细胞，用蒸馏水洗涤，并用于负染色标本制备。
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