[细胞培养中支原体检测方法敏感性的比较研究]

[Comparative study of the sensitivity of Mycoplasma detection methods in cell cultures].

作者信息

Shcheglovitova O N, Etkind G V, Lotte V D, Strochkova L S, Bektemirov T A

出版信息

Vopr Virusol. 1982 Jul-Aug;27(4):456-61.

Abstract

The results of a comparative study of the sensitivity of several methods for mycoplasma detection in cell cultures are presented. The most sensitive method was found to be that of electron microscopy detecting mycoplasmal contamination in 100% preparations. Seeding of the material on mycoplasma-elective nutrient medium (0.3% PPLO agar) and the method of autoradiography detect the culture contamination in 90% of the test materials. Staining of cell cultures with orsein and Schiff reagent are less sensitive methods revealing mycoplasmal contamination in 69% and 77.7% of the cultures, respectively.

摘要

本文展示了几种检测细胞培养物中支原体方法的敏感性比较研究结果。发现最敏感的方法是电子显微镜法，该方法在100%的制剂中检测到支原体污染。将材料接种于支原体选择性营养培养基（0.3%PPLO琼脂）上以及放射自显影法在90%的测试材料中检测到培养物污染。用玫瑰红和席夫试剂对细胞培养物进行染色是较不敏感的方法，分别在69%和77.7%的培养物中检测到支原体污染。