巴氏芽孢梭菌铁氧化还原蛋白的质子磁共振研究。
Proton magnetic resonance study of ferredoxin from Clostridium pasteurianum.
作者信息
Poe M, Phillips W D, McDonald C C, Lovenberg W
出版信息
Proc Natl Acad Sci U S A. 1970 Apr;65(4):797-804. doi: 10.1073/pnas.65.4.797.
Abstract
Magnetic susceptibilities of both reduced and oxidized ferredoxin from Clostridium pasteurianum were obtained in solution. Whereas the reduced form exhibits a Curie law behavior, the magnetic susceptibility of oxidized ferredoxin in fact increases with temperature and suggests extensive antiferromagnetic exchange coupling between the component iron atoms. Contact-shifted resonances are observed for both forms of ferredoxin that are attributed to the beta-CH(2) protons of the eight cysteine residues. A model based on these results is presented.
摘要
测定了巴氏梭菌还原型和氧化型铁氧化还原蛋白在溶液中的磁化率。还原型呈现居里定律行为，而氧化型铁氧化还原蛋白的磁化率实际上随温度升高，这表明其组成铁原子之间存在广泛的反铁磁交换耦合。两种形式的铁氧化还原蛋白均观察到接触位移共振，这归因于八个半胱氨酸残基的β-CH(2)质子。基于这些结果提出了一个模型。
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