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巴氏芽孢梭菌铁氧化还原蛋白的质子磁共振研究。

Proton magnetic resonance study of ferredoxin from Clostridium pasteurianum.

作者信息

Poe M, Phillips W D, McDonald C C, Lovenberg W

出版信息

Proc Natl Acad Sci U S A. 1970 Apr;65(4):797-804. doi: 10.1073/pnas.65.4.797.

DOI:10.1073/pnas.65.4.797
PMID:5266908
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC282985/
Abstract

Magnetic susceptibilities of both reduced and oxidized ferredoxin from Clostridium pasteurianum were obtained in solution. Whereas the reduced form exhibits a Curie law behavior, the magnetic susceptibility of oxidized ferredoxin in fact increases with temperature and suggests extensive antiferromagnetic exchange coupling between the component iron atoms. Contact-shifted resonances are observed for both forms of ferredoxin that are attributed to the beta-CH(2) protons of the eight cysteine residues. A model based on these results is presented.

摘要

测定了巴氏梭菌还原型和氧化型铁氧化还原蛋白在溶液中的磁化率。还原型呈现居里定律行为，而氧化型铁氧化还原蛋白的磁化率实际上随温度升高，这表明其组成铁原子之间存在广泛的反铁磁交换耦合。两种形式的铁氧化还原蛋白均观察到接触位移共振，这归因于八个半胱氨酸残基的β-CH(2)质子。基于这些结果提出了一个模型。

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本文引用的文献

1
An electron transport factor from Clostridium pasteurianum.来自巴氏梭菌的一种电子传递因子。
Biochem Biophys Res Commun. 1962 Jun 4;7:448-52. doi: 10.1016/0006-291x(62)90333-9.
2
AN X-RAY INVESTIGATION OF THE STRUCTURE OF A BACTERIAL FERREDOXIN.细菌铁氧化还原蛋白结构的X射线研究
Biochem Biophys Res Commun. 1965 Jun 18;20:33-5. doi: 10.1016/0006-291x(65)90945-9.
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THE NATURE OF IRON IN FERREDOXIN.铁氧化还原蛋白中铁的性质。
Proc Natl Acad Sci U S A. 1964 Jun;51(6):1085-92. doi: 10.1073/pnas.51.6.1085.
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STUDIES ON THE CHEMICAL NATURE OF CLOSTRIDIAL FERREDOXIN.梭菌铁氧化还原蛋白化学性质的研究。
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Manifestations of the tertiary structures of proteins in high-frequency nuclear magnetic resonance.蛋白质三级结构在高频核磁共振中的表现
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Additional observations on the chemistry of clostridial ferredoxin.关于梭菌铁氧化还原蛋白化学性质的更多观察结果。
Biochemistry. 1966 Apr;5(4):1262-8. doi: 10.1021/bi00868a020.
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DSS as an activator of carboxypeptidase A.作为羧肽酶A激活剂的二硫代琥珀酸（DSS）
Biochem Biophys Res Commun. 1969 Jul 7;36(1):126-30. doi: 10.1016/0006-291x(69)90658-5.
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Proton magnetic resonance spectra of proteins in random-coil configurations.随机卷曲构象的蛋白质的质子磁共振谱。
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Paramagnetic proton nuclear magnetic resonance shifts of metmyoglobin, methemoglobin, and hemin derivatives.高铁肌红蛋白、高铁血红蛋白和血红素衍生物的顺磁质子核磁共振位移。
J Am Chem Soc. 1968 May 8;90(10):2700-1. doi: 10.1021/ja01012a048.
10
The amino acid sequence of Clostridium pasteurianum ferredoxin.巴氏芽孢梭菌铁氧化还原蛋白的氨基酸序列。
Biochemistry. 1966 May;5(5):1666-81. doi: 10.1021/bi00869a032.