高结合比例下十二烷基硫酸盐与蛋白质的结合。对生物膜中蛋白质状态的可能影响。
Binding of dodecyl sulfate to proteins at high binding ratios. Possible implications for the state of proteins in biological membranes.
作者信息
Reynolds J A, Tanford C
出版信息
Proc Natl Acad Sci U S A. 1970 Jul;66(3):1002-7. doi: 10.1073/pnas.66.3.1002.
Abstract
A wide variety of proteins have been shown to bind identical amounts of an amphiphile, sodium dodecyl sulfate, on a gram per gram basis at monomer equilibrium concentrations above 0.5 mM. The binding is independent of ionic strength and primarily hydrophobic in nature. Only the monomeric form of the amphiphile binds to proteins, not the micellar form. The application of these results to models for biological membranes and to gel electrophoresis in sodium dodecyl sulfate is discussed.
摘要
已表明，在单体平衡浓度高于0.5 mM时，多种蛋白质能以每克蛋白质结合相同量两亲物十二烷基硫酸钠。这种结合与离子强度无关，本质上主要是疏水作用。只有两亲物的单体形式能与蛋白质结合，而非胶束形式。本文讨论了这些结果在生物膜模型和十二烷基硫酸钠凝胶电泳中的应用。
相似文献
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Binding of dodecyl sulfate to proteins at high binding ratios. Possible implications for the state of proteins in biological membranes.高结合比例下十二烷基硫酸盐与蛋白质的结合。对生物膜中蛋白质状态的可能影响。
Proc Natl Acad Sci U S A. 1970 Jul;66(3):1002-7. doi: 10.1073/pnas.66.3.1002.
2
The gross conformation of protein-sodium dodecyl sulfate complexes.蛋白质 - 十二烷基硫酸钠复合物的总体构象。
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本文引用的文献
1
THE PHYSICAL PROPERTIES OF A PROTEIN ISOLATED FROM BACTERIOPHAGE PHI-X174.从噬菌体φ-X174中分离出的一种蛋白质的物理性质。
J Mol Biol. 1963 Oct;7:388-400. doi: 10.1016/s0022-2836(63)80032-7.
2
The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes.人红细胞无血红蛋白空泡的制备及其化学特性
Arch Biochem Biophys. 1963 Jan;100:119-30. doi: 10.1016/0003-9861(63)90042-0.
3
Formation of membranes by repeating units.由重复单元形成膜。
Arch Biochem Biophys. 1967 Mar;119(1):312-35. doi: 10.1016/0003-9861(67)90461-4.
4
On the ionic strength dependence of micelle number. II.论胶束数量对离子强度的依赖性。II.
J Phys Chem. 1967 May;71(6):1898-907. doi: 10.1021/j100865a057.
5
The binding of divers detergent anions to bovine serum albumin.不同洗涤剂阴离子与牛血清白蛋白的结合。
Biochemistry. 1967 Mar;6(3):937-47. doi: 10.1021/bi00855a038.
6
Binding of large organic anions and neutral molecules by native bovine serum albumin.天然牛血清白蛋白对大有机阴离子和中性分子的结合作用。
Biochemistry. 1966 Aug;5(8):2606-16. doi: 10.1021/bi00872a019.
7
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
8
Association of lipids and proteins in chloroplast lamellar membrane.叶绿体片层膜中脂质与蛋白质的关联
Biochim Biophys Acta. 1968 Jun 11;150(4):686-93. doi: 10.1016/0005-2736(68)90058-8.
9
Current models for the structure of biological membranes.生物膜结构的当前模型。
J Cell Biol. 1969 Sep;42(3):613-46. doi: 10.1083/jcb.42.3.613.
10
Protein denaturation.蛋白质变性
Adv Protein Chem. 1968;23:121-282. doi: 10.1016/s0065-3233(08)60401-5.
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