肽和蛋白质的可控脱酰胺作用：一种实验风险及可能的生物定时器。
Controlled deamidation of peptides and proteins: an experimental hazard and a possible biological timer.
作者信息
Robinson A B, McKerrow J H, Cary P
出版信息
Proc Natl Acad Sci U S A. 1970 Jul;66(3):753-7. doi: 10.1073/pnas.66.3.753.
Abstract
Experiments on model peptides show that the rate of deamidation of asparaginyl residues depends strongly on the nature of neighboring residues. The natural distribution of glutaminyl and asparaginyl residues is ordered with respect to the biological lifetime of the peptides and the functional groups of the residues neighboring to glutaminyl and asparaginyl residues. The rates of deamidation of such amide peptides under physiological conditions could serve as useful timers of development and aging.
摘要
对模型肽的实验表明，天冬酰胺残基的脱酰胺速率强烈依赖于相邻残基的性质。谷氨酰胺残基和天冬酰胺残基的自然分布在肽的生物寿命以及与谷氨酰胺和天冬酰胺残基相邻的残基官能团方面是有序的。此类酰胺肽在生理条件下的脱酰胺速率可作为发育和衰老的有用计时指标。
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本文引用的文献
1
The determination of glutamine in the presence of asparagine.在天冬酰胺存在的情况下谷氨酰胺的测定。
Biochem J. 1935 Dec;29(12):2710-20. doi: 10.1042/bj0292710.
2
The estimation of glutamine in the presence of asparagine.在天冬酰胺存在的情况下对谷氨酰胺的测定。
Biochem J. 1932;26(1):122-32. doi: 10.1042/bj0260122.
3
Biosynthesis of ribonuclease in mouse pancreas.小鼠胰腺中核糖核酸酶的生物合成。
J Biol Chem. 1960 May;235:1404-8.
4
Turnover of rat-liver mitochondria.大鼠肝脏线粒体的周转
Biochim Biophys Acta. 1961 Aug 5;51:356-60. doi: 10.1016/0006-3002(61)90177-9.
5
The proteins of bovine pancreatic juice. II. Rates of synthesis in vivo of the cationic proteins.牛胰液中的蛋白质。II. 阳离子蛋白质的体内合成速率。
J Biol Chem. 1959 Feb;234(2):311-5.
6
The metabolism of myosin, the meromyosins, actin and tropomyosin in the rabbit.兔体内肌球蛋白、肌球蛋白亚基、肌动蛋白和原肌球蛋白的代谢
Biochim Biophys Acta. 1956 Apr;20(1):228-36. doi: 10.1016/0006-3002(56)90281-5.
7
Use of hydrogen fluoride in Merrifield solid-phase peptide synthesis.氟化氢在梅里菲尔德固相肽合成中的应用。
J Am Chem Soc. 1967 Jan 4;89(1):181-2. doi: 10.1021/ja00977a057.
8
9
The synthesis of bovine insulin by the solid phase method.用固相法合成牛胰岛素。
J Am Chem Soc. 1966 Nov 5;88(21):5051-2. doi: 10.1021/ja00973a068.
10
On the heterogeneity of beef heart cytochrome c. 3. A kinetic study of the non-enzymic deamidation of the main subfractions (Cy I-Cy 3).论牛心细胞色素c的异质性。3. 主要亚组分（Cy I - Cy 3）非酶促脱酰胺作用的动力学研究。
Acta Chem Scand. 1966;20(6):1487-96. doi: 10.3891/acta.chem.scand.20-1487.
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