BALB/c小鼠中针对异源肾小球和肾小管基底膜抗体的特异性
Specificity of antibodies to heterologous glomerular and tubular basement membranes in BALB/c mice.
作者信息
Erard D, Moulonguet-Doleris D, Auffredou M T, Graindorge P, Mahieu P, Galanaud P, Dormont J
出版信息
Clin Exp Immunol. 1979 Nov;38(2):259-64.
Balb/c mice were immunized with dog insoluble glomerular basement membrane (GBM) or tubular basement membrane (TBM). The titre of circulating antibodies was sequentially determined and their specificity was analysed using various soluble membrane fractions. Glomerular and tubular deposits were studied on serial biopsies by direct immunofluorescence. After elution, from whole kidneys, IgG fixation on normal mouse kidney sections was analysed by indirect immunofluorescence. The conclusions are the following: (a) after immunization with insoluble GBM, the antibodies are mainly directed against collagenous antigenic determinants shared by GBM and TBM; (b) after immunization with insoluble TBM, the antibodies are mainly directed against noncollagenous TBM-specific antigenic determinants.
用犬不溶性肾小球基底膜（GBM）或肾小管基底膜（TBM）免疫Balb/c小鼠。依次测定循环抗体的滴度，并使用各种可溶性膜组分分析其特异性。通过直接免疫荧光对连续活检组织中的肾小球和肾小管沉积物进行研究。洗脱后，通过间接免疫荧光分析全肾中IgG在正常小鼠肾脏切片上的固定情况。得出以下结论：（a）用不溶性GBM免疫后，抗体主要针对GBM和TBM共有的胶原抗原决定簇；（b）用不溶性TBM免疫后，抗体主要针对非胶原性TBM特异性抗原决定簇。
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