Moulonguet-Doleris D, Erard D, Auffredou M T, Foidart J M, Mahieu P, Dormont J
Clin Exp Immunol. 1981 Oct;46(1):35-43.
C3H, CBA (H-2k) and NZB (H-2d) mice were immunized with dog insoluble glomerular (GBM) or tubular basement membrane (TBM). The titre of circulating antibodies was sequentially determined and their specificity was analysed using various soluble antigenic fractions. Glomerular and tubular deposits were studied on serial biopsies by direct immunofluorescence. After elution from whole kidneys, IgG fixation on normal mouse kidney sections was analysed by indirect immunofluorescence. After immunization with insoluble GBM, animals from all three strains develop antibodies mainly directed against collagenous antigenic determinants shared by GBM and TBM. After immunization with insoluble TBM, the antibodies are directed in NZB mice against non-collagenous TBM-specific determinants, in C3H mice against collagenous determinants and in CBA mice against both types of antigenic determinants. Thus the ability to respond to the various antigens of GBM and TBM is genetically determined and does not depend only on the major histocompatibility complex.
用犬不溶性肾小球基底膜（GBM）或肾小管基底膜（TBM）对C3H、CBA（H-2k）和NZB（H-2d）小鼠进行免疫。依次测定循环抗体的滴度，并使用各种可溶性抗原组分分析其特异性。通过直接免疫荧光对连续活检组织中的肾小球和肾小管沉积物进行研究。从全肾洗脱后，通过间接免疫荧光分析IgG在正常小鼠肾切片上的固定情况。用不溶性GBM免疫后，所有三个品系的动物均产生主要针对GBM和TBM共有的胶原抗原决定簇的抗体。用不溶性TBM免疫后，NZB小鼠的抗体针对非胶原性TBM特异性决定簇，C3H小鼠的抗体针对胶原性决定簇，CBA小鼠的抗体针对两种类型的抗原决定簇。因此，对GBM和TBM各种抗原的反应能力是由基因决定的，并不只取决于主要组织相容性复合体。
