Richmond R C, Powell J R
Proc Natl Acad Sci U S A. 1970 Nov;67(3):1264-7. doi: 10.1073/pnas.67.3.1264.
A wild population of Drosophila paulistorum (Andean semispecies) was analyzed by starch gel electrophoresis for a sex-linked enzyme locus, Tetrazolium oxidase. The results of the analysis of F(1) progenies from 106 single female lines from São José do Rio Prêto, Brazil, has allowed us to determine the genotype of 106 wild-collected females and 104 wild-collected males. The population is polymorphic for two alleles with frequencies of 0.56 and 0.44. The observed frequencies among the females of the two homozygotes were 0.23 and 0.13 and of the heterozygote 0.64. The deviation from the frequencies expected according to the Hardy-Weinberg formula is statistically significant (P < 0.005). There is a substantial excess of heterozygotes over the expected number. This observation is interpreted to mean that this polymorphism is maintained by heterosis. This is probably the first reported case of heterosis associated with an enzyme locus in a natural population of Drosophila.
通过淀粉凝胶电泳对野生的圣保罗果蝇（安第斯半种）群体进行了分析，以研究一个性连锁酶基因座——四唑氧化酶。对来自巴西里约普雷图河畔圣若泽的106个单雌系的F(1)后代进行分析的结果，使我们能够确定106只野生采集的雌性和104只野生采集的雄性的基因型。该群体对于两个等位基因呈多态性，其频率分别为0.56和0.44。在雌性中观察到的两种纯合子的频率分别为0.23和0.13，杂合子的频率为0.64。与根据哈迪-温伯格公式预期的频率的偏差具有统计学意义（P < 0.005）。杂合子的数量大大超过预期数量。这一观察结果被解释为意味着这种多态性是由杂种优势维持的。这可能是首次报道的与果蝇自然群体中一个酶基因座相关的杂种优势案例。
