Sherwood L M, Rodman J S, Lundberg W B
Proc Natl Acad Sci U S A. 1970 Nov;67(3):1631-8. doi: 10.1073/pnas.67.3.1631.
Bovine parathyroid glands incubated in organ culture synthesize and secrete parathyroid hormone in response to physiologic alterations in cation concentration. Hormone synthesized in the glands is immunologically different and higher in molecular weight than that released into the culture medium or circulating in bovine blood, although it is identical to purified hormone extracted from the glands. On the basis of the physiologic responses of tissue and medium hormone to cation, the transfer of radioactive label from one moiety to another, and interconversion of the two forms by a gland homogenate, it is suggested that the parathyroid glands synthesize a precursor hormone (proparathormone) that is modified to the smaller molecule that circulates in blood.
在器官培养中孵育的牛甲状旁腺会根据阳离子浓度的生理变化合成并分泌甲状旁腺激素。腺体中合成的激素在免疫学上与释放到培养基中或在牛血液中循环的激素不同，分子量也更高，尽管它与从腺体中提取的纯化激素相同。根据组织和培养基激素对阳离子的生理反应、放射性标记从一个部分转移到另一个部分以及腺体匀浆对两种形式的相互转化，有人提出甲状旁腺合成一种前体激素（甲状旁腺素原），该前体激素会被修饰成在血液中循环的较小分子。
