Kiefer H, Lindstrom J, Lennox E S, Singer S J
Proc Natl Acad Sci U S A. 1970 Dec;67(4):1688-94. doi: 10.1073/pnas.67.4.1688.
Acetylcholinesterase of intact red blood cell membranes and the acetylcholine receptor at the neuromuscular junction of whole-frog sartorius muscle have been irreversibly inactivated by photo-affinity labeling with two quaternary ammonium aryl azides. The inactivation requires that the azides, at the time of their photolytic conversion to highly reactive nitrenes, are reversibly bound to the specific acetylcholine-binding sites.
完整红细胞膜的乙酰胆碱酯酶以及全蛙缝匠肌神经肌肉接头处的乙酰胆碱受体,已被两种季铵芳基叠氮化物通过光亲和标记不可逆地失活。失活要求叠氮化物在光解转化为高活性氮烯时,能可逆地结合到特定的乙酰胆碱结合位点上。