Baenziger N L, Brodie G N, Majerus P W
Proc Natl Acad Sci U S A. 1971 Jan;68(1):240-3. doi: 10.1073/pnas.68.1.240.
The action of thrombin on intact human platelets has been studied with the aid of polyacrylamide gel electrophoresis in sodium dodecylsulfate. A single major membrane protein band with a molecular weight of 190,000 disappears after thrombin treatment, while a new membrane protein with a molecular weight of 107,000 appears. This may represent hydrolysis of the thrombin-sensitive protein. When platelets are disrupted or when the thrombin-sensitive protein is solubilized from membranes prior to thrombin treatment, no hydrolysis occurs. The effect of thrombin on the platelet membrane protein is complete within 2 min which suggests that hydrolysis of this membrane protein may trigger the physiological effects of thrombin on platelets.
借助十二烷基硫酸钠聚丙烯酰胺凝胶电泳，对凝血酶作用于完整的人血小板的情况进行了研究。凝血酶处理后，一条分子量为190,000的主要膜蛋白带消失，同时出现一条分子量为107,000的新膜蛋白。这可能代表凝血酶敏感蛋白的水解。当血小板被破坏时，或者在凝血酶处理前将凝血酶敏感蛋白从膜上溶解时，不会发生水解。凝血酶对血小板膜蛋白的作用在2分钟内完成，这表明该膜蛋白的水解可能触发凝血酶对血小板的生理作用。
