Hakim A A
Exp Cell Biol. 1979;47(5):332-50. doi: 10.1159/000162953.
The effect of treatment with trypsin and neuraminidase on morphology, proliferation pattern, adhesiveness and ultrastructure of human normal mammary and carcinoma cell lines has been investigated. These criteria were chosen because they reflect on the cell surface function. Active enzymes were required to produce changes. If trypsinized and then allowed to grow in culture media supplemented with 'fibroblast growth-promoting factor', human normal mammary epithelial cells proliferated in a disorganized pattern with cells overlapping and piling up and developing ultrastructural characteristics of neoplastic cells. On the other hand, if treated with neuraminidase and then permitted to grow in presence of the 'fibroblast growth-promoting factor', mammary carcinoma cells proliferated in an organized pattern, forming one-cell-thick, fibroblast-like monolayers and ultrastructural characteristics of normal epithelial cells.
已研究了用胰蛋白酶和神经氨酸酶处理对人正常乳腺和癌细胞系的形态、增殖模式、黏附性及超微结构的影响。选择这些标准是因为它们反映了细胞表面功能。需要活性酶来产生变化。如果将人正常乳腺上皮细胞用胰蛋白酶处理，然后在补充有“成纤维细胞生长促进因子”的培养基中培养，细胞会以无序模式增殖，细胞相互重叠、堆积，并呈现肿瘤细胞的超微结构特征。另一方面，如果用神经氨酸酶处理，然后在“成纤维细胞生长促进因子”存在的情况下培养，乳腺癌细胞会以有序模式增殖，形成单层的、成纤维细胞样的单细胞厚层，并呈现正常上皮细胞的超微结构特征。
