Yang N S, Kirkland W, Jorgensen T, Furmanski P
J Cell Biol. 1980 Jan;84(1):120-30. doi: 10.1083/jcb.84.1.120.
Primary monolayer cultures of normal and malignant human mammary epithelial cells were tested for fibronectin by indirect immunofluorescence using antisera specific for fibronectin. This protein was not detectable on either the normal or malignant epithelial cells. Similar results were obtained for normal and malignant mouse mammary epithelial cell cultures. Control normal and transformed fibroblasts exhibited the expected result: the normal cells were positive and the transformed cells were negative. With the use of supernatant fluids from the same cultures or an agar-overlay assay on viable cells, high levels of plasminogen-dependent fibrinolytic activity were detectable in both the normal and malignant mammary cells. Thus, two characteristics that distinguish normal from transformed fibroblasts do not serve as markers of malignancy in mammary epithelial/carcinoma systems.
使用针对纤连蛋白的抗血清，通过间接免疫荧光法对正常和恶性人乳腺上皮细胞的原代单层培养物进行纤连蛋白检测。在正常或恶性上皮细胞上均未检测到这种蛋白质。正常和恶性小鼠乳腺上皮细胞培养物也得到了类似结果。对照正常和成纤维细胞表现出预期结果：正常细胞呈阳性，转化细胞呈阴性。使用来自相同培养物的上清液或对活细胞进行琼脂覆盖试验，在正常和恶性乳腺细胞中均检测到高水平的纤溶酶原依赖性纤维蛋白溶解活性。因此，区分正常和成纤维细胞的两个特征在乳腺上皮/癌系统中并非恶性肿瘤的标志物。
