Brawn R J
Proc Natl Acad Sci U S A. 1971 Jul;68(7):1634-8. doi: 10.1073/pnas.68.7.1634.
The possibility that lymphocytes sensitized to cell-associated antigens may be rendered hyporeactive toward cells expressing these antigens by prior incubation with such antigens in soluble form was investigated. Mice were sensitized to the foreign histocompatibility antigens of an allogeneic strain of mice; lymphocytes from such sensitized mice were incubated with serum from normal mice of the sensitizing, sensitized, and F(1) hybrids of the two strains. These lymphocytes were washed and their in vitro activity against preparations of cells of sensitizing strain origin was measured by modifications of a standard assay. Sera from the sensitizing strain, and from F(1) hybrids of the sensitizing and sensitized strains (which would be expected to contain the soluble histocompatibility antigens to which the lymphocytes were sensitized), abrogated the in vitro activity of lymphocytes against target cells of the sensitizing strain, while serum from the sensitized strain did not. Soluble tumor-specific or histocompatibility antigens may be responsible in part for the specific abrogation of sensitized lymphocyte activity by serum from tumorbearing, successfully allografted, allogeneically pregnant, or chimeric animals.
研究了对细胞相关抗原致敏的淋巴细胞，是否可能因事先与可溶性形式的此类抗原孵育，而对表达这些抗原的细胞反应性降低。将小鼠对同种异体小鼠品系的外来组织相容性抗原致敏；将来自此类致敏小鼠的淋巴细胞与致敏品系、致敏后小鼠以及两品系的F(1)杂种正常小鼠的血清一起孵育。洗涤这些淋巴细胞，并通过对标准检测方法的改进，来测定它们对致敏品系来源的细胞制剂的体外活性。致敏品系以及致敏品系与致敏后小鼠的F(1)杂种（预期含有淋巴细胞致敏的可溶性组织相容性抗原）的血清，消除了淋巴细胞对致敏品系靶细胞的体外活性，而致敏后小鼠的血清则没有。可溶性肿瘤特异性或组织相容性抗原可能部分导致了荷瘤、成功接受同种异体移植、同种异体妊娠或嵌合动物血清对致敏淋巴细胞活性的特异性消除。
