Alexander A M, Gonda I, Harpur E S, Kayes J B
J Antibiot (Tokyo). 1979 May;32(5):504-10. doi: 10.7164/antibiotics.32.504.
Microelectrophoresis was found to be a rapid method for studying the interaction between aminoglycoside antibiotics and liposomes prepared from acid phospholipids. The ability to cause charge reversal of liposomes prepared from phosphatidyl inositol was ranked in the order neomycin greater than gentamicin greater than tobramycin greater than amikacin greater than kanamycin greater than ribostamycin greater than streptomycin greater than dihydrostreptomycin greater than Ca2+. Similar results were obtained with liposomes prepared from a mixture (8: 2) of phosphatidyl choline and phosphatidic acid, but no effect was detectable with neutral liposomes made from phosphatidyl choline only. The results support the view that the attraction between positively charged nitrogen groups on the antibiotics and the negatively charged groups of acidic phospholipids are predominantly responsible for the interaction. Extension of the studies to ionic strengths and calcium concentrations similar to those found in vivo showed a reduction, but not elimination, of the observed effects.
微电泳被发现是一种研究氨基糖苷类抗生素与由酸性磷脂制备的脂质体之间相互作用的快速方法。使由磷脂酰肌醇制备的脂质体发生电荷反转的能力排序为：新霉素＞庆大霉素＞妥布霉素＞阿米卡星＞卡那霉素＞核糖霉素＞链霉素＞二氢链霉素＞Ca²⁺。由磷脂酰胆碱和磷脂酸的混合物（8:2）制备的脂质体也得到了类似结果，但仅由磷脂酰胆碱制成的中性脂质体未检测到任何影响。这些结果支持这样一种观点，即抗生素上带正电的氮基团与酸性磷脂的带负电基团之间的吸引力是这种相互作用的主要原因。将研究扩展到与体内相似的离子强度和钙浓度时，观察到的效应有所降低，但并未消除。
