Reyonolds A V, Hamilton-Miller J M, Brumfitt W
J Infect Dis. 1976 Nov;134 SUPPL:S291-6. doi: 10.1093/infdis/135.supplement_2.s291.
Sixty-nine strains of gentamicin-resistant gram-negative bacilli obtained from different geographical sources were tested for susceptibility to 11 aminoglycoside antibiotics. From the results of determinations of minimal inhibitory concentrations, patterns of resistance were established for 45 strains of Enterobacteriaceae and 24 strains of Pseudomonas aeruginosa. Overall, 81% of the strains were sensitive to amikacin and 33% of the strains were sensitive to butirosin, the next most active compound. Results indicated that 54% of the P. aeruginosa strains were sensitive to amikacin and 33% were sensitive to tobramycin. From resistance patterns, enzymes responsible for inactivation of the antibiotics were deduced. The most common enzyme was aminoglycoside nucleotidyltransferase(2''), either alone or combined with either aminoglycoside phosphotransferase(3')-I or aminoglycoside phosphotransferase(3')-II. Aminoglycoside acetyltransferase(2) was identified exclusively in strains of Providencia stuartii. Specific enzymes could not be identified for 30 strains, 21 of which were P. aeruginosa.
对从不同地理来源分离得到的69株耐庆大霉素革兰氏阴性杆菌进行了11种氨基糖苷类抗生素的药敏试验。根据最低抑菌浓度测定结果，确定了45株肠杆菌科细菌和24株铜绿假单胞菌的耐药模式。总体而言，81%的菌株对阿米卡星敏感，33%的菌株对下一个活性最强的化合物布替罗星敏感。结果表明，54%的铜绿假单胞菌菌株对阿米卡星敏感，33%对妥布霉素敏感。根据耐药模式推断出负责抗生素失活的酶。最常见的酶是氨基糖苷核苷酸转移酶(2'')，单独存在或与氨基糖苷磷酸转移酶(3')-I或氨基糖苷磷酸转移酶(3')-II联合存在。氨基糖苷乙酰转移酶(2)仅在斯氏普罗威登斯菌菌株中鉴定到。30株菌株无法鉴定出特定的酶，其中21株为铜绿假单胞菌。
