Daniels C R, Swan E P
J Chromatogr Sci. 1979 Nov;17(11):628-30. doi: 10.1093/chromsci/17.11.628.
A solution of internal standard in acetonitrile is used to extract samples of wood from lumber which had been commercially treated with chlorinated phenols. The tetrachlorophenol(TCP) and pentachlorophenol( PCP) were analytically separated from each other and from the other wood extractive compounds using a high performance liquid chromatograph (HPLC). The ultraviolet (UV) absorptions of the TCP, PCP, and internal standard were automatically measured as they eluted. The UV absorption peaks were integrated, and the amounts of TCP and PCP present were calculated with a dedicated microcomputer. Compared with the method previously used, this method is faster (up to 144 samples per 48 hours), has the same accuracy and precision, and it is linear over the concentration range used.
乙腈中的内标溶液用于从经商业氯化酚处理的木材中提取木材样本。使用高效液相色谱仪（HPLC）将四氯苯酚（TCP）和五氯苯酚（PCP）彼此分离，并与其他木材提取物化合物分离。TCP、PCP和内标的紫外（UV）吸收在洗脱时自动测量。对UV吸收峰进行积分，并使用专用微型计算机计算存在的TCP和PCP的量。与先前使用的方法相比，该方法更快（每48小时最多可处理144个样本），具有相同的准确度和精密度，并且在所使用的浓度范围内呈线性。
