Kalman D A
J Chromatogr Sci. 1984 Oct;22(10):452-5. doi: 10.1093/chromsci/22.10.452.
A method for the determination of pentachlorophenol, 2,3,4,6-tetrachlorophenol, and their salts or hydrolyzable biological conjugates in urine, water, serum, or fish tissue is reported. The method utilizes fused silica capillary gas chromatography (GC) with electron capture detection of the free phenols following hydrolysis and extraction into toluene. A recovery standard (4-chloro-2-nitrophenol) and an internal standard for GC (1-nitro-2,3,5,6-tetrachlorobenzene) are used to monitor recovery and GC performance. The detection limit for TCP and PCP is 0.5 ppb and the precision observed for each sample in the 1- to 100-ppb range is between 3 and 12% relative standard deviation for both components.
本文报道了一种测定尿液、水、血清或鱼组织中五氯苯酚、2,3,4,6-四氯苯酚及其盐类或可水解生物共轭物的方法。该方法采用熔融石英毛细管气相色谱法（GC），通过电子捕获检测水解后并萃取至甲苯中的游离酚类。使用回收标准品（4-氯-2-硝基苯酚）和气相色谱内标物（1-硝基-2,3,5,6-四氯苯）来监测回收率和气相色谱性能。TCP和PCP的检测限为0.5 ppb，在1至100 ppb范围内，每个样品中两种成分的相对标准偏差在3%至12%之间。
