• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

小鼠核糖体RNA外部转录间隔区的加工及放线菌素D的作用位点

Processing of the external transcribed spacer of murine rRNA and site of action of actinomycin D.

作者信息

Fetherston J, Werner E, Patterson R

出版信息

Nucleic Acids Res. 1984 Sep 25;12(18):7187-98. doi: 10.1093/nar/12.18.7187.

DOI:10.1093/nar/12.18.7187
PMID:6091060
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC320150/
Abstract

The primary rRNA transcript contains a large external transcribed spacer (ETS) approximately 4,000 nucleotides in length. We have used subcloned DNA probes derived from the 5' end of the ETS in conjunction with Northern blot analysis of murine nuclear RNA to examine processing of this region. In agreement with the results of previous investigators, we find that the large rRNA precursor lacks part of the ETS region. These ETS sequences are also missing from subsequent rRNA processing intermediates. Experiments using actinomycin D confirm that the excision of portion of the ETS is an early event in rRNA processing. In addition, in the presence of actinomycin D small RNA species accumulate which hybridize to a probe specific for the 5' end of the ETS. The length of these abbreviated transcripts defines a region of rDNA which is probably a target for this drug.

摘要

初级rRNA转录本包含一个长度约为4000个核苷酸的大的外部转录间隔区（ETS）。我们使用了源自ETS 5'端的亚克隆DNA探针，并结合对小鼠核RNA的Northern印迹分析来研究该区域的加工过程。与先前研究者的结果一致，我们发现大的rRNA前体缺少部分ETS区域。这些ETS序列在随后的rRNA加工中间体中也不存在。使用放线菌素D的实验证实，ETS部分的切除是rRNA加工中的早期事件。此外，在放线菌素D存在的情况下，会积累与ETS 5'端特异性探针杂交的小RNA种类。这些缩短转录本的长度定义了rDNA的一个区域，该区域可能是这种药物的作用靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6eb0/320150/ec1b06379aae/nar00336-0276-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6eb0/320150/90d20332f62d/nar00336-0274-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6eb0/320150/ec1b06379aae/nar00336-0276-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6eb0/320150/90d20332f62d/nar00336-0274-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6eb0/320150/ec1b06379aae/nar00336-0276-a.jpg

相似文献

1
Processing of the external transcribed spacer of murine rRNA and site of action of actinomycin D.小鼠核糖体RNA外部转录间隔区的加工及放线菌素D的作用位点
Nucleic Acids Res. 1984 Sep 25;12(18):7187-98. doi: 10.1093/nar/12.18.7187.
2
Complex endonucleolytic cleavage pattern during early events in the processing of pre-rRNA in the lower eukaryote, Tetrahymena thermophila.嗜热四膜虫这种低等真核生物前体核糖体RNA加工早期事件中的复杂核酸内切酶切割模式。
Nucleic Acids Res. 1983 Jun 11;11(11):3487-502. doi: 10.1093/nar/11.11.3487.
3
Actinomycin D stimulates the transcription of rRNA minigenes transfected into mouse cells. Implications for the in vivo hypersensitivity of rRNA gene transcription.放线菌素D刺激转染到小鼠细胞中的rRNA小基因的转录。对rRNA基因转录体内超敏反应的影响。
Eur J Biochem. 1995 Mar 15;228(3):605-15. doi: 10.1111/j.1432-1033.1995.0605m.x.
4
Characterization of pre-rRNA components in ribosomal precursor particles from macronuclei of Tetrahymena thermophila.嗜热四膜虫大核核糖体前体颗粒中前体rRNA成分的表征
Eur J Cell Biol. 1989 Aug;49(2):225-35.
5
Spacer sequences downstream of the 28S RNA coding region are part of the mouse rDNA transcription unit.28S RNA编码区下游的间隔序列是小鼠rDNA转录单元的一部分。
Nucleic Acids Res. 1985 Apr 11;13(7):2293-304. doi: 10.1093/nar/13.7.2293.
6
Nucleotide sequence of an external transcribed spacer in Xenopus laevis rDNA: sequences flanking the 5' and 3' ends of 18S rRNA are non-complementary.非洲爪蟾核糖体DNA中外转录间隔区的核苷酸序列：18S核糖体RNA 5'和3'末端侧翼的序列不互补。
Nucleic Acids Res. 1982 Apr 10;10(7):2387-98. doi: 10.1093/nar/10.7.2387.
7
tRNA genes are found between 16S and 23S rRNA genes in Bacillus subtilis.在枯草芽孢杆菌中，转运RNA基因位于16S和23S核糖体RNA基因之间。
Nucleic Acids Res. 1982 Mar 11;10(5):1607-24. doi: 10.1093/nar/10.5.1607.
8
Localization of small nuclear polymerase I RNA sequences at the 5' end of the human rDNA transcription unit.
EMBO J. 1984 Feb;3(2):473-9. doi: 10.1002/j.1460-2075.1984.tb01831.x.
9
U3 small nuclear RNA can be psoralen-cross-linked in vivo to the 5' external transcribed spacer of pre-ribosomal-RNA.U3小核RNA在体内可被补骨脂素交联到核糖体前体RNA的5'外部转录间隔区。
Proc Natl Acad Sci U S A. 1989 Sep;86(17):6523-7. doi: 10.1073/pnas.86.17.6523.
10
Organization of ribosomal RNA gene repeats of the mouse.小鼠核糖体RNA基因重复序列的组织
Nucleic Acids Res. 1981 Jul 24;9(14):3219-33. doi: 10.1093/nar/9.14.3219.

引用本文的文献

1
9-Aminoacridine Inhibits Ribosome Biogenesis by Targeting Both Transcription and Processing of Ribosomal RNA.9-氨基吖啶通过靶向核糖体 RNA 的转录和加工来抑制核糖体生物发生。
Int J Mol Sci. 2022 Jan 23;23(3):1260. doi: 10.3390/ijms23031260.
2
Ribosomal proteins and human diseases: molecular mechanisms and targeted therapy.核糖体蛋白与人类疾病：分子机制与靶向治疗。
Signal Transduct Target Ther. 2021 Aug 30;6(1):323. doi: 10.1038/s41392-021-00728-8.
3
FGFR2 mutations in bent bone dysplasia syndrome activate nucleolar stress and perturb cell fate determination.

本文引用的文献

1
Transcription of mouse rRNA genes by RNA polymerase I: in vitro and in vivo initiation and processing sites.RNA聚合酶I对小鼠rRNA基因的转录：体外和体内的起始及加工位点
Cell. 1981 Nov;27(1 Pt 2):165-74. doi: 10.1016/0092-8674(81)90370-6.
2
Multiple ribosomal RNA cleavage pathways in mammalian cells.哺乳动物细胞中的多种核糖体RNA切割途径。
Nucleic Acids Res. 1981 Oct 10;9(19):4951-66. doi: 10.1093/nar/9.19.4951.
3
Biogenesis of ribosomes in eukaryotes.真核生物中核糖体的生物合成
弯曲骨发育不良综合征中的FGFR2突变激活核仁应激并扰乱细胞命运决定。
Hum Mol Genet. 2017 Sep 1;26(17):3253-3270. doi: 10.1093/hmg/ddx209.
4
5'-end surveillance by Xrn2 acts as a shared mechanism for mammalian pre-rRNA maturation and decay.Xrn2 通过 5' 端监控作用作为哺乳动物前 rRNA 成熟和降解的共同机制。
Nucleic Acids Res. 2011 Mar;39(5):1811-22. doi: 10.1093/nar/gkq1050. Epub 2010 Oct 29.
5
Polyadenylation and degradation of incomplete RNA polymerase I transcripts in mammalian cells.真核生物细胞中 RNA 聚合酶 I 不完全转录本的多聚腺苷酸化和降解。
EMBO Rep. 2010 Feb;11(2):106-11. doi: 10.1038/embor.2009.271. Epub 2010 Jan 8.
6
Early stages of pre-rRNA formation within the nucleolar ultrastructure of mouse cells studied by in situ hybridization with a 5'ETS leader probe.通过用5'ETS前导探针进行原位杂交研究小鼠细胞核仁超微结构中前体rRNA形成的早期阶段。
Chromosoma. 1997 Jun;105(7-8):496-505. doi: 10.1007/BF02510486.
7
Processing in the external transcribed spacer of ribosomal RNA from Physarum polycephalum.多头绒泡菌核糖体RNA外部转录间隔区的加工
Nucleic Acids Res. 1986 Apr 25;14(8):3153-66. doi: 10.1093/nar/14.8.3153.
8
Characterization of ribosomal RNA synthesis in a gene dosage mutant: the relationship of topoisomerase I and chromatin structure to transcriptional activity.基因剂量突变体中核糖体RNA合成的特征：拓扑异构酶I和染色质结构与转录活性的关系。
J Cell Biol. 1987 Oct;105(4):1501-13. doi: 10.1083/jcb.105.4.1501.
9
RNA metabolism in nuclei: adenovirus and heat shock alter intranuclear RNA compartmentalization.细胞核中的RNA代谢：腺病毒和热休克改变核内RNA的区室化。
Nucleic Acids Res. 1989 Mar 11;17(5):2081-98. doi: 10.1093/nar/17.5.2081.
10
RNA metabolism in nuclei: selective transport of kappa exons from myeloma nuclei and adenoviral transcripts from infected HeLa nuclei.细胞核中的RNA代谢：骨髓瘤细胞核中κ外显子的选择性转运以及感染的HeLa细胞核中腺病毒转录本的选择性转运。
Nucleic Acids Res. 1986 May 27;14(10):4159-69. doi: 10.1093/nar/14.10.4159.
Subcell Biochem. 1980;7:1-80. doi: 10.1007/978-1-4615-7948-9_1.
4
Isolation and partial characterization of the Drosophila alcohol dehydrogenase gene.果蝇乙醇脱氢酶基因的分离与部分特性分析
Proc Natl Acad Sci U S A. 1980 Oct;77(10):5794-8. doi: 10.1073/pnas.77.10.5794.
5
Location of the initial cleavage sites in mouse pre-rRNA.小鼠前体核糖体RNA中初始切割位点的位置
Mol Cell Biol. 1983 Aug;3(8):1501-10. doi: 10.1128/mcb.3.8.1501-1510.1983.
6
In vitro transcription of a cloned mouse ribosomal RNA gene.克隆的小鼠核糖体RNA基因的体外转录
Nucleic Acids Res. 1981 Dec 21;9(24):6773-85. doi: 10.1093/nar/9.24.6773.
7
Mapping of a mouse ribosomal DNA promoter by in vitro transcription.通过体外转录对小鼠核糖体DNA启动子进行定位
Nucleic Acids Res. 1981 Nov 25;9(22):6093-102. doi: 10.1093/nar/9.22.6093.
8
The nucleotide sequence of the initiation region of the ribosomal transcription unit from mouse.来自小鼠的核糖体转录单位起始区域的核苷酸序列。
Nucleic Acids Res. 1981 Apr 10;9(7):1559-69. doi: 10.1093/nar/9.7.1559.
9
The bidirectional transfer of DNA and RNA to nitrocellulose or diazobenzyloxymethyl-paper.DNA和RNA向硝酸纤维素或重氮苄氧基甲基纸的双向转移。
Anal Biochem. 1980 Nov 15;109(1):123-9. doi: 10.1016/0003-2697(80)90019-6.
10
The nucleotide sequence of the putative transcription initiation site of a cloned ribosomal RNA gene of the mouse.小鼠克隆核糖体RNA基因推定转录起始位点的核苷酸序列。
Nucleic Acids Res. 1980 Dec 20;8(24):6043-58. doi: 10.1093/nar/8.24.6043.