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28S RNA编码区下游的间隔序列是小鼠rDNA转录单元的一部分。

Spacer sequences downstream of the 28S RNA coding region are part of the mouse rDNA transcription unit.

作者信息

Grummt I, Sorbaz H, Hofmann A, Roth E

出版信息

Nucleic Acids Res. 1985 Apr 11;13(7):2293-304. doi: 10.1093/nar/13.7.2293.

DOI:10.1093/nar/13.7.2293
PMID:2582362
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC341156/
Abstract

Evidence is presented that more than 300 bp of spacer sequences downstream of the 28S RNA coding sequence are part of the mouse rDNA transcription unit. Studies in two cell-free transcription systems as well as analysis of cellular RNA indicate that RNA polymerase I does not terminate within the 334 bp 3' terminal spacer sequences contained in the rDNA clone used. Quantitative hybridization data, S1 mapping experiments and Northern analysis of nuclear RNA showed that the 14 kb pre-rRNA molecules hybridize with the same efficiency to both the 28S and the 3' NTS specific DNA probe. This indicates that the rRNA precursor contains both at the 5' and 3' end several hundreds bases of external transcribed spacer sequences which are eliminated in subsequent processing reactions.

摘要

有证据表明，28S RNA编码序列下游超过300 bp的间隔序列是小鼠rDNA转录单元的一部分。在两种无细胞转录系统中的研究以及对细胞RNA的分析表明，RNA聚合酶I不会在所使用的rDNA克隆中包含的334 bp 3'末端间隔序列内终止。定量杂交数据、S1图谱实验和核RNA的Northern分析表明，14 kb的前体rRNA分子与28S和3' NTS特异性DNA探针的杂交效率相同。这表明rRNA前体在5'和3'末端都包含数百个外部转录间隔序列的碱基，这些碱基在随后的加工反应中被去除。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6138/341156/b1d32081f383/nar00301-0124-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6138/341156/763a48f86694/nar00301-0119-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6138/341156/a2480e71b478/nar00301-0121-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6138/341156/96c4388881b7/nar00301-0123-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6138/341156/b1d32081f383/nar00301-0124-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6138/341156/763a48f86694/nar00301-0119-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6138/341156/a2480e71b478/nar00301-0121-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6138/341156/96c4388881b7/nar00301-0123-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6138/341156/b1d32081f383/nar00301-0124-a.jpg

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Faithful in vivo transcription termination of Xenopus laevis rDNA. Correlation of electron microscopic spread preparations with S1 transcript analysis.非洲爪蟾核糖体DNA在体内的忠实转录终止。电子显微镜铺展制备与S1转录本分析的相关性。
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The nucleotide sequence at the transcription termination site of the ribosomal RNA gene in Tetrahymena thermophila.嗜热四膜虫核糖体RNA基因转录终止位点的核苷酸序列。
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Mapping of a mouse ribosomal DNA promoter by in vitro transcription.通过体外转录对小鼠核糖体DNA启动子进行定位
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