Grim M, Carlson B M
J Embryol Exp Morphol. 1979 Dec;54:99-111.
The purpose of this experiment was to examine the relationship, if any, between nerve fibers and the formation of muscle pattern in the regenerating amphibian limb. During embryogenesis, nerve fibers grow into the limb bud at the time when the common muscle blastemas subdivide into individual muscle primordia, whereas in regeneration nerve fibers are always present. In order to learn whether or not the muscle pattern could be laid down in the absence of nerves we amputated 58 limbs of newts (Notophthalmus viridescens) at the mid humeral level and allowed them to regenerate to the medium-bud or late-bud stage. The limbs were then denervated. The majority of limbs denervated at the medium-bud stage either regressed or failed to regenerate further. Regeneration after denervation failed in 9 of 25 limbs denervated at the late-bud stages. In those limbs that continued to regenerate after denervation, the formation of individual muscle primordia did occur, following the same sequence with respect to the gross stage of regeneration as innervated regenerates. In comparing these results with our previous results on the development of muscular pattern in aneurogenic limbs of the axolotl, we conclude that in neither the embryonic nor the regenerating amphibian limb are nerve fibers directly involved in the subdivision of common muscle blastemas into the primordia of individual muscles.
本实验的目的是研究神经纤维与再生两栖动物肢体肌肉模式形成之间是否存在关系。在胚胎发育过程中，当共同的肌芽分化为单个肌肉原基时，神经纤维长入肢芽，而在再生过程中神经纤维始终存在。为了了解在没有神经的情况下肌肉模式是否能够形成，我们在肱骨中部水平切断了58只蝾螈（绿红东美螈）的肢体，并让它们再生至中芽或晚芽阶段。然后对这些肢体进行去神经处理。大多数在中芽阶段去神经的肢体要么退化，要么无法进一步再生。在晚芽阶段去神经的25只肢体中，有9只去神经后再生失败。在那些去神经后继续再生的肢体中，确实发生了单个肌肉原基的形成，就再生的总体阶段而言，其顺序与有神经支配的再生肢体相同。将这些结果与我们之前关于美西螈无神经肢体肌肉模式发育的结果进行比较后，我们得出结论，在胚胎期或再生期的两栖动物肢体中，神经纤维都不直接参与共同肌芽分化为单个肌肉原基的过程。
