McLaughlin H M, Rathbone M P, Liversage R A, McLaughlin D S
J Exp Zool. 1983 Feb;225(2):175-85. doi: 10.1002/jez.1402250202.
The effect of short-term denervation (0, 12, 24, and 72 hours) on the levels of cyclic 3'5'-guanosine monophosphate (cGMP) and cyclic 3'5'-adenosine monophosphate (cAMP) in adult newt (Notophthalmus viridescens) forelimbs at 15, 22, and 35 days of regeneration was investigated. Regenerate blastema and stump cyclic nucleotide levels were compared with those of the contralateral intact forelimb and hindlimb, with levels in the normally regenerating blastema, and with levels measured in the forelimbs of intact, nonoperated animals. Variations in cyclic nucleotide levels occurred according to regeneration stage and tissue type. Changes in level were noted immediately upon denervation and subsequently at other sample times in all regenerate and control series. Parallel fluctuations occurred in regenerate stump and contralateral intact forelimbs. Our results from nonamputated denervated and sham-denervated animals indicate that short-term, denervation-associated cyclic nucleotide fluctuations cannot be attributed solely to the loss of innervation.
研究了短期去神经支配（0、12、24和72小时）对成年蝾螈（绿红东美螈）前肢在再生15、22和35天时的环磷酸鸟苷（cGMP）和环磷酸腺苷（cAMP）水平的影响。将再生芽基和残端的环核苷酸水平与对侧完整前肢和后肢的水平、正常再生芽基中的水平以及未手术的完整动物前肢中测得的水平进行了比较。环核苷酸水平根据再生阶段和组织类型而变化。去神经支配后立即以及随后在所有再生和对照系列的其他采样时间都记录到了水平变化。再生残端和对侧完整前肢出现了平行波动。我们对未截肢的去神经支配和假去神经支配动物的研究结果表明，与去神经支配相关的短期环核苷酸波动不能仅仅归因于神经支配的丧失。
