Robertson H D, Lodish H F
Proc Natl Acad Sci U S A. 1970 Oct;67(2):710-6. doi: 10.1073/pnas.67.2.710.
The proteins initiated in vitro by nascent bacteriophage f2 RNA strands attached to isolated replicating structures have been analyzed. The observations confirm that coat protein is the major product initiated and completed. Nascent strands direct the initiation of viral maturation protein in amounts similar to the maximum levels observed in vivo; this synthesis is independent of translation of the coat protein gene. However, only a fraction of these maturation protein molecules initiated in vitro is completed. Nascent RNA molecules also direct the initiation of appreciable amounts of viral RNA polymerase protein, very little of which is completed. Certain constraints on the in vitro translation of the polymerase gene from single-stranded RNA appear to be relaxed in the nascent strands, as indicated by the reduced effect of a polar amber mutation in the coat cistron upon polymerase protein initiation from nascent RNA.
对附着于分离的复制结构上的新生噬菌体f2 RNA链在体外起始合成的蛋白质进行了分析。观察结果证实，外壳蛋白是起始合成并完成合成的主要产物。新生链指导病毒成熟蛋白的起始合成，其合成量与体内观察到的最大水平相似；这种合成独立于外壳蛋白基因的翻译。然而，在体外起始合成的这些成熟蛋白分子中只有一部分能够完成合成。新生RNA分子也指导相当数量的病毒RNA聚合酶蛋白的起始合成，其中很少一部分能够完成合成。如外壳顺反子中的极性琥珀突变对新生RNA起始合成聚合酶蛋白的影响减弱所示，新生链似乎放松了对单链RNA上聚合酶基因体外翻译的某些限制。
