Edelman G M, Rutishauser U, Millette C F
Proc Natl Acad Sci U S A. 1971 Sep;68(9):2153-7. doi: 10.1073/pnas.68.9.2153.
A new method, fiber fractionation, has been used to isolate and separate cells. The cells are adsorbed to fibers covalently coupled to molecules such as antigens, antibodies, and lectins which can bind specifically to cell-surface components. The cells are then removed mechanically by plucking the taut fibers. Alternatively, competitive inhibitors of binding may be used to remove the cells at a lesser rate. Successful fractionations have been achieved by varying the degree of derivatization of the fibers by the lectin concanavalin A. Lymphoid cells have been separated by the use of different antigens coupled to the fibers. The method may also be used for specific fixation and manipulation of viable cell populations in culture. In addition to fibers, beads and surfaces have been specifically derivatized and used to achieve different geometrical arrangements of the cells.
一种新方法——纤维分级分离法，已被用于分离和区分细胞。细胞被吸附到与抗原、抗体和凝集素等分子共价偶联的纤维上，这些分子能特异性结合细胞表面成分。然后通过拔紧绷的纤维将细胞机械去除。或者，结合的竞争性抑制剂可用于以较低速率去除细胞。通过改变伴刀豆球蛋白A对纤维的衍生化程度已成功实现分级分离。利用与纤维偶联的不同抗原已分离出淋巴细胞。该方法还可用于培养中有活力细胞群体的特异性固定和操作。除纤维外，珠子和表面也已被特异性衍生化，并用于实现细胞的不同几何排列。
