致畸剂体内和体外活性之间的定量对应关系。
Quantitative correspondence between the in vivo and in vitro activity of teratogenic agents.
作者信息
Braun A G, Buckner C A, Emerson D J, Nichinson B B
出版信息
Proc Natl Acad Sci U S A. 1982 Mar;79(6):2056-60. doi: 10.1073/pnas.79.6.2056.
Abstract
We have tested 74 teratogenic and 28 nonteratogenic agents in a recently developed in vitro teratogen assay system. The assay identifies teratogens by their ability to inhibit attachment of ascites tumor cells to plastic surfaces coated with concanavalin A. There is a qualitative agreement between in vivo animal data and in vitro activity for 81 of the 102 agents (79%). Quantitative analysis shows a highly significant correlation coefficient of 0.69 between the inhibitory in vitro dose and the lowest reported teratogenic dose for 54 of the 60 inhibitory teratogens. The doses analyzed ranged over 5 orders of magnitude. We interpret these results to mean that attachment inhibition in concert with other, complementary, in vitro assay systems can become a useful method for the assessment of the teratogenic potential of environmental agents.
摘要
我们在最近开发的体外致畸剂检测系统中对74种致畸剂和28种非致畸剂进行了测试。该检测通过致畸剂抑制腹水肿瘤细胞附着于包被伴刀豆球蛋白A的塑料表面的能力来识别致畸剂。102种试剂中的81种（79%）在体内动物数据和体外活性之间存在定性一致性。定量分析显示，60种抑制性致畸剂中，54种的体外抑制剂量与报告的最低致畸剂量之间的相关系数高达0.69。分析的剂量范围跨越5个数量级。我们将这些结果解释为，结合其他互补的体外检测系统的附着抑制可以成为评估环境试剂致畸潜力的有用方法。
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