Kasamatsu H, Robberson D L, Vinograd J
Proc Natl Acad Sci U S A. 1971 Sep;68(9):2252-7. doi: 10.1073/pnas.68.9.2252.
A fraction of the covalently closed mitochondrial DNA in mouse L cells contains a replicated heavy-strand segment that is hydrogen bonded to the circular light strand. The inserted single strand is dissociable from the circular duplex at an elevated temperature.
小鼠L细胞中一小部分共价闭合的线粒体DNA含有一个复制的重链片段,该片段通过氢键与环状轻链相连。在升高的温度下,插入的单链可从环状双链体上解离。