Wolff R K, Silbaugh S A, Brownstein D G, Carpenter R L, Mauderly J L
J Toxicol Environ Health. 1979 Nov;5(6):1037-47. doi: 10.1080/15287397909529812.
To determine mortality caused by inhaled sulfuric acid mist, groups of Hartley guinea pigs 2-3 mo old were exposed for 8 h to graded concentrations of aerosols of 0.4 or 0.8 microm mass median aerodynamic diameter. Relative humidity during exposures was maintained at 70-80%. Based on probit analysis, the concentration required to produce 50% mortality (LC50) for deaths to 21 d after exposure was 30 mg/m3 for the 0.8-microm aerosol. For the 0.4-microm aerosol, the LC50 was above 109 mg/m3, the highest concentration obtainable at that particle size. At both particle sizes, the animals either tended to develop severe dyspnea and die minutes (0.4 microm) or hours (0.8 microm) thereafter or appeared nearly unaffected. Lesions in animals that died as a results of 0.4-microm exposures were restricted to hyperinflation; animals that died as a result of 0.8-microm exposures also showed hemorrhage and transudation. No gross or histopathologic changes were observed in animals that appeared unaffected during exposure. Differences in total and/or regional respiratory tract deposition may account for the different responses to the two aerosols.
为了确定吸入硫酸雾所致的死亡率，将2至3月龄的Hartley豚鼠分组，使其暴露于质量中值空气动力学直径为0.4或0.8微米的不同浓度气溶胶中8小时。暴露期间的相对湿度维持在70%-80%。基于概率分析，对于暴露后21天内死亡而言，产生50%死亡率（LC50）所需的0.8微米气溶胶浓度为30毫克/立方米。对于0.4微米气溶胶，LC50高于109毫克/立方米，这是该粒径可达到的最高浓度。在两种粒径情况下，动物要么趋于出现严重呼吸困难，并在数分钟（0.4微米）或数小时（0.8微米）后死亡，要么几乎未受影响。因暴露于0.4微米气溶胶而死亡的动物病变仅限于肺过度充气；因暴露于0.8微米气溶胶而死亡的动物还出现出血和渗出。在暴露期间未受影响的动物中未观察到大体或组织病理学变化。总呼吸道和/或局部呼吸道沉积的差异可能解释了对两种气溶胶的不同反应。
