O'Brien J R, Heywood J B
J Clin Pathol. 1967 Jan;20(1):56-64. doi: 10.1136/jcp.20.1.56.
If native or heparinized blood is passed slowly through a column of glass beads at room temperature, the number of platelets removed from the initial drop emerging from the column is less than that removed from the final drop. At 4 degrees C. this difference disappears. If the blood is passed rapidly through such columns at room temperature fewer platelets are removed, but the initial-final difference persists. Von Willebrand's platelets are removed normally at slow speeds; at fast speeds abnormally few platelets are removed. Platelets emerging from all such columns are in aggregates. On adding glass beads to normal heparinized plasma, the platelets at once become more rounded and after about 50 seconds' delay they aggregate: the delay and rate of aggregation can be quantitated. Aggregation occurs best at 20 to 30 degrees C. and is not inhibited by the addition of some enzyme inhibitors. In von Willebrand's disease all these glass-induced aggregation phenomena occur normally and aggregation in response to adenosine diphosphate (ADP), serotonin creatinine sulphate (5-HT), adrenaline, collagen, and glass is also normal.
如果将未处理的或肝素化的血液在室温下缓慢通过一列玻璃珠，从柱中流出的最初一滴血液中被去除的血小板数量比最后一滴中的少。在4摄氏度时，这种差异消失。如果血液在室温下快速通过此类柱，则被去除的血小板较少，但最初与最后的差异仍然存在。血管性血友病患者的血小板在慢速时正常被去除；在快速时被异常少量地去除。从所有此类柱中流出的血小板都聚集成团。向正常肝素化血浆中加入玻璃珠后，血小板立即变得更圆，约50秒后延迟聚集：这种延迟和聚集速率可以定量。聚集在20至30摄氏度时最佳，并且不受某些酶抑制剂添加的抑制。在血管性血友病中，所有这些玻璃诱导的聚集现象都正常发生，并且对二磷酸腺苷（ADP）、5-羟色胺硫酸肌酐（5-HT）、肾上腺素、胶原蛋白和玻璃的聚集反应也正常。
