通过补偿照明波动来改进显微光度测量法。
Improved microphotometry by compensating for fluctuations in the illumination.
作者信息
Kronberg H, Zimmer H G, Neuhoff V
出版信息
Microsc Acta. 1979 Nov;82(3):223-8.
PMID:530093
Abstract
Behind a beam-splitter in the illuminator, the irradiation of an area conjugated to the object plane is measured by a silicon photodiode and used as a reference signal. Analog division of the photomulitplier signal by this reference acts like stabilizing the light source by a factor greater than 25. A technical realization of the optical and electronical components as well as experimental results are described.
摘要
在照明器中的一个分束器后面，与物平面共轭的区域的照射由一个硅光电二极管测量，并用作参考信号。用该参考信号对光电倍增管信号进行模拟除法，其作用类似于将光源稳定一个大于25的系数。描述了光学和电子组件的技术实现以及实验结果。
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