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通过补偿照明波动来改进显微光度测量法。

Improved microphotometry by compensating for fluctuations in the illumination.

作者信息

Kronberg H, Zimmer H G, Neuhoff V

出版信息

Microsc Acta. 1979 Nov;82(3):223-8.

PMID:530093
Abstract

Behind a beam-splitter in the illuminator, the irradiation of an area conjugated to the object plane is measured by a silicon photodiode and used as a reference signal. Analog division of the photomulitplier signal by this reference acts like stabilizing the light source by a factor greater than 25. A technical realization of the optical and electronical components as well as experimental results are described.

摘要

在照明器中的一个分束器后面,与物平面共轭的区域的照射由一个硅光电二极管测量,并用作参考信号。用该参考信号对光电倍增管信号进行模拟除法,其作用类似于将光源稳定一个大于25的系数。描述了光学和电子组件的技术实现以及实验结果。

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