Boldt M, Harbig K, Weidemann G, Lübbers D W
Pflugers Arch. 1980 May;385(2):167-73. doi: 10.1007/BF00588698.
The construction of a microscope photometer using prefabricated elements is described. To illuminate the tissue, a Leitz Ultropac is applied. To enlarge the wavelength range, its illuminating glass lens is replaced by an Acryl glass zonal lens. Two separate light channels with separate lamps, monochromators and photomultipliers allow the measurement of fluorescence excitation and emission spectra as well as of reflection spectra. By chopping the light, light pulses and dark currents are measured 8.33 times a second. By an integration circuit the signal-to-noise ratio for small signals is improved. The instrument allows us to detect the increase of 4 ng/ml NADH (solution of pH 7.38) in an area of 0.2 mm2.
本文描述了一种使用预制元件构建的显微镜光度计。为了照亮组织，使用了徕卡Ultropac。为了扩大波长范围，其照明玻璃透镜被丙烯酸玻璃带状透镜所取代。两个独立的光通道，分别配备灯、单色仪和光电倍增管，可用于测量荧光激发光谱、发射光谱以及反射光谱。通过对光进行斩波，每秒可测量8.33次光脉冲和暗电流。通过积分电路，可提高小信号的信噪比。该仪器能够检测出在0.2平方毫米的区域内4纳克/毫升烟酰胺腺嘌呤二核苷酸（pH值为7.38的溶液）的增加。
